Transcriptomics

Dataset Information

29

Detecting transcription profile associated to mammosphere medium


ABSTRACT: Mammosphere medium (Grange et al. Neoplasia 2008;10(12):1433-43) contains epidermal growth factor (EGF), insulin and basic fibroblast growth factor (bFGF) that may influence transcript expression in a way not necessarily related to the assembly of mammspheres. To identify the set of genes associated mammosphere cell growth medium we analyzed two prototypic situations in triplicate experiments: TUBO cells (a mouse breast cancer cell line derived by Balb-neuT mice) grown in DMEM under adherent conditions (TDA), TUBO cells grown in Mammosphere medium under adherent conditions (TMA). Overall design: Two prototypic situations in triplicate experiments were analysed: TUBO cells (Rovero et al. Gene Ther 2001;8(6):447-52) grown in DMEM under adherent conditions (TDA), TUBO cells grown in Mammosphere medium under adherent conditions (TMA).

INSTRUMENT(S): Illumina MouseWG-6 v2.0 expression beadchip

ORGANISM(S): Mus musculus  

SUBMITTER: Raffaele A Calogero  

PROVIDER: GSE26517 | GEO |

SECONDARY ACCESSION(S): PRJNA136705

REPOSITORIES: GEO

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