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Temperature dependent gene expression levels at 28°C and 37°C for rhizophere Rhizosphere Originating Biocontrol Strain Pseudomonas aeruginosa M18

ABSTRACT: To further understand the gene expression characteristics of originating biocontrol strain Pseudomonas aeruginosa M18, we have applied whole genome microarray expression profiling as a discovery platform to to specify the temperature dependent expression of M18 genome at rhizosphere and human body temperature. We selected 28°C as temperature representative of the rhizosphere niches and 37°C for human body. The results from the temperature dependent transcriptome analysis are consistent to our previous published data that the phzM, ptsP and lasI genes expression is upregulated at 37°C. The comparison analysis of the M18 genome expressional profiles at 28°C and 37°C indicated a total of 605 gene expressed in a temperature dependent manner over about two fold at 28°C compared that at 37°C, covering 10.6% genes in M18 whole genome.

ORGANISM(S): Pseudomonas sp. M18 Pseudomonas aeruginosa

PROVIDER: GSE26518 | GEO | 2012/01/01

SECONDARY ACCESSION(S): PRJNA136707

REPOSITORIES: GEO

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Temperature dependent gene expression levels at 28°C and 37°C for rhizophere Rhizosphere Originating Biocontrol Strain Pseudomonas aeruginosa M18

Project description:To further understand the gene expression characteristics of originating biocontrol strain Pseudomonas aeruginosa M18, we have applied whole genome microarray expression profiling as a discovery platform to to specify the temperature dependent expression of M18 genome at rhizosphere and human body temperature. We selected 28°C as temperature representative of the rhizosphere niches and 37°C for human body. The results from the temperature dependent transcriptome analysis are consistent to our previous published data that the phzM, ptsP and lasI genes expression is upregulated at 37°C. The comparison analysis of the M18 genome expressional profiles at 28°C and 37°C indicated a total of 605 gene expressed in a temperature dependent manner over about two fold at 28°C compared that at 37°C, covering 10.6% genes in M18 whole genome. Cells were grown to OD600=5.0-6.0 (late exponential phase) in LB medium at 28℃ and 37℃, respectively. Three independent experiments were performed at each time.

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Temperature dependent gene expression levels at 28°C and 37°C for Pseudomonas aeruginosa PAO1

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Project description:To further understand the gene expression characteristics of originating biocontrol strain Pseudomonas aeruginosa M18, we have applied whole genome microarray expression profiling as a discovery platform to to specify the PCA-dependent expression of M18 genome. We constructed a series of PCA-producing mutant strains (high PCA: M18MSU1; low PCA: M18MS; and no PCA: M18MSP1P2). The comparison analysis of the M18 mutants genome expressional profiles indicated that the expression of PCA in both M18MSU1 and M18MS alters the expression of a total of 545 different genes; however, the higher level of PCA in M18MSU1 altered more genes (489) as compared to M18MS (129). Cells were grown to OD600=5.0 (exponential phase) in PPM medium at 28℃. Three independent experiments were performed at each time.

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