Developmental scRNA-seq data set of a sea urchin Hemicentrotus pulcherrimus

ABSTRACT: We employed the Western Pacific model sea urchin, Hemicentrotus pulcherrimus, as our experimental system to establish a comprehensive single-cell RNA sequencing (scRNA-seq) data atlas. Our primary objective was to document stage-specific gene expression profiles during the normal developmental progression, ranging from 24 to 96 hours post-fertilization. Our particular focus lay on neurogenesis, a pivotal process occurring in the latter stages of sea urchin development. To elucidate the regulatory mechanisms governing neurogenesis, we utilized pharmacological interventions targeting the Delta-Notch signaling pathway, a central regulator of this process. By perturbing the pathway's lateral inhibition from 16 hours post-fertilization, we aimed to identify gene sets typically suppressed during neurogenesis. Subsequent to the inhibition, we observed a notable upregulation of neurogenic genes and a concurrent expansion in the cellular population expressing these genes, starting from the 24-hour time point. In addition, the processed Seurat objects (RDS files), stratified by developmental stage, experimental condition, and sample, are available through the HpHase portal (https://cell-innovation.nig.ac.jp/Hpul/).

ORGANISM(S): Hemicentrotus pulcherrimus

PROVIDER: GSE265747 | GEO | 2025/10/08

REPOSITORIES: GEO