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Evaluating Aquaporin-5 (Aqp5) functions in mouse pyloric cancer stem cells

ABSTRACT: To study Aqp5 functions in pyloric cancer stem cells, we isolated GFP+ putative stem cells and GFP- tumour epithelial cells from Aqp5 wildtype and Aqp5 knockout pyloric mouse tumours to understand the functions of Aqp5 in these cancer stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE265919 | GEO | 2025/09/23

REPOSITORIES: GEO

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Evaluating Aquaporin-5 (Aqp5) functions in mouse pyloric cancer stem cells

Project description:Evaluating Aquaporin-5 (Aqp5) functions in mouse pyloric cancer stem cells

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Project description:Isolating adult stem cells from human stomach epithelia has proven challenging due to a lack of surface markers that are amenable to antibody-based sorting approaches. Here, through comparative expression profiling of mouse Lgr5+ adult stem cells along the gastrointestinal tract, we identify novel pylorus-specific stem cell markers, including the membrane protein Aqp5. FACS-based isolation of endogenous AQP5+ cells from healthy human pyloric stomach revealed their stem cell identity in organoid assays. Using new Aqp5-driven CreERT2 mouse models to selectively target conditional mutations to the pyloric stem cell compartment in vivo, we establish pyloric stem cells as a major source of Wnt-driven invasive gastric cancer. The new stem cell markers and mouse models described here will be an invaluable resource for deciphering early gastric cancer formation and for isolating and characterizing human stomach stem cells as a prerequisite to potentially exploiting their regenerative medicine potential in the clinic.

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