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Transcription Activator FgDDT Interacts with FgISW1 to Regulates Hyphal Development and Virulence in Fusarium graminearum

ABSTRACT: The Imitation Switch (ISWI) chromatin remodeling factors are known to form stable remodeling complexes with members from a family of proteins, termed DDT-domain proteins. ISWIs have been reported to be involved in various biological processes in many eukaryotic species. However, the regulatory mechanisms of DDT-domain proteins in ISWI complexes remain unclear. In this study, using ChIP-seq assays, we found that FgDDT binds to the promoter of MAPK and growth-related gene, activating its expression. Moreover, we identified and characterized two additional ISWI genes, FgISW1 and FgISW2, indicating that the ISWI components FgISW1 and FgDDT, rather than FgISW2, are essential for fungal development and virulence. Subsequently, we illustrated that FgDDT binds to FgISW1 to form an ISWI complex that activates the expression of functional genes in Fusarium graminearum, contributing to the virulence and development of F. graminearum. We also we observed that FgDDT is highly conserved in Fusarium species, but exhibits low similarity in Homo sapiens and Arabidopsis thaliana. These findings provide a basis for further studies on the molecular mechanisms by which ISWI complexes function in fungi.

ORGANISM(S): Fusarium graminearum PH-1

PROVIDER: GSE265948 | GEO | 2025/04/03

REPOSITORIES: GEO

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