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Omics score: 20

Transcriptome profiling of LecRKVI.2 over-expressor plants.

ABSTRACT: The arabidopsis L-type lectin receptor kinase-VI.2 positively regulates bacterial PAMP-triggered immunity. We used microarrays and performed genome-wide transcriptome analysis of LecRKVI.2 over-expressing plants and identified number of upregulated genes involved against bacterial resistance. Overall design: Total RNA was isolated from 5-week-old Arabidopsis plants for RNA extraction and hybridisation on Affymetrix microarrays.

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: PRASHANT SINGH

PROVIDER: GSE26646 | GEO | 2012-03-19

SECONDARY ACCESSION(S): PRJNA136377

REPOSITORIES: GEO

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Similar Datasets

Transcriptome profiling of LecRKVI.2 over-expressor plants.

Project description:The arabidopsis L-type lectin receptor kinase-VI.2 positively regulates bacterial PAMP-triggered immunity. We used microarrays and performed genome-wide transcriptome analysis of LecRKVI.2 over-expressing plants and identified number of upregulated genes involved against bacterial resistance. Total RNA was isolated from 5-week-old Arabidopsis plants for RNA extraction and hybridisation on Affymetrix microarrays.

2012-03-19 | E-GEOD-26646 | ArrayExpress

Transcriptome profiling of LecRKVI.2 over-expressor plants.

2015-11-20 | E-GEOD-26646 | ExpressionAtlas

Transcript profiling upon ethanol-induced expression of Xcv type 3- effector xopB in A. thaliana

Project description:The bacterial pathogen Xanthomonas campestris pv. vesicatoria 85-10 (Xcv) translocates about 30 type 3 effector proteins (T3Es) into pepper plants (Capsicum annuum) to suppress its immune responses. Among them is XopB which was previously shown to interfere with cell wall-bound invertase (CwInv) and sugar-mediated defence responses. Transgenic Arabidopsis thaliana lines expressing xopB under control of an ethanol inducible promotor were generated to investigate the in planta activity of this effector protein in more depth. The microarray analysis revealed that expression of the bacterial T3E XopB in A. thaliana plants causes immediate and massive reprogramming of transcription resulting in the alteration of the hormonal homeostasis and imbalances in signalling cascades. Further analyses unravelled that XopB modifies the pathogen associated molecular pattern (PAMP)-triggered calcium signature and inhibits the burst of reactive oxygen species (ROS). Overall design: For microarray analyses, five week old transgenic plants (# 10, 12, 27) and wild-type plants were watered with 10 ml 1% (v/v) ethanol per pot after light has switch on in the climate chamber. Leaves from individual plants were harvested 4 h after treatment and immediately frozen in liquid nitrogen. Development of phenotypic changes was monitored and leaves of plants showing comparable phenotypes were pooled into 3 replicates.

| GSE66477 | GEO

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae. Overall design: Gene expression in leaves of Nicotiana attenuata wild type and ir-lecRK1 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-lecRK1 plants. A total of 148 genes were identified as differentially regulated in ir-lecRK1 plants compared to wild type plants.

| GSE29905 | GEO

Early ETI and PTI responses in Arabidopsis thaliana camta3-D (sr1-4d) mutant

Project description:Innate immune responses of plant cells confer the first line of defence against pathogens. Signals generated by activated receptors are integrated inside the cell and converge on transcriptional programmes in the nucleus. In Arabidopsis, the CAMTA family of transcription factors plays a pivotal function in immunity. CAMTA binding motifs are highly enriched in the genes quickly induced during ETI and PTI. Using RNA-seq, we investigated the role of CAMTA TFs during the early ETI and PTI transcriptional responses. Overall design: We compared the expression changes between an Arabidopsis mutant line carrying the camta3-D (sr1-4d) dominant negative mutation and Col-0 wild-type plants following treatment with the PAMP flg22 (100 nM), and the avirulent bacterial strains Pst DC3000 AvrRpm1 (OD600=0.001) and Pst DC3000 AvrRps4 (OD600=0.001). The expression changes were analysed at one time point after each treatment (1 hour for flg22 and 4 hours for Pst DC3000 AvrRpm1 and Pst DC3000 AvrRps4 treatments).

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Transcription profiling by high throughput sequencing of Arabidopsis transgenic plants overexpressing HBI1 gene

Project description:The trade-off between growth and immunity is crucial for survival in plants. An antagonistic interaction has been observed between the growth-promoting hormone brassinosteroid and pathogen associated molecular pattern (PAMP) signals, which induce immunity but inhibit growth, however the underlying molecular mechanism has remained unclear. The PRE-IBH1-HBI1 triple helix-loop-helix/basic helix-loop-helix (HLH/bHLH) cascade has been shown to mediate growth responses to several hormonal and environmental signals, but its downstream targets and role in immunity remain unknown. Here, we performed genome-wide analyses of HBI1 target genes in Arabidopsis. The results show that HBI1 regulates a set of genes that largely overlaps with targets of PIFs, but displays both similar and unique transcriptional activities compared to PIFs, supporting a role in fine-tuning the network through cooperation and antagonism with other DNA-binding factors of the network. Furthermore, HBI1 also negatively regulates a subset of defense response genes. Two PAMPs, flagellin and elongation factor, repressed HBI1 expression, whereas overexpression of HBI1 reduced the PAMP-induced growth inhibition, defense gene expression, reactive oxygen species (ROS) production, and flg22-induced resistance to Pseudomonas syringae pathovar tomato DC3000. These data indicate that HBI1 is a node for crosstalk between hormone and immune pathways. This study demonstrates that the PRE-IBH1-HBI1 module integrates hormone and pathogen signals, and thus plays a central role in the balance between growth and immunity in plants. Compare the transcriptome of HBI1-Ox and wild type.

2014-05-30 | E-GEOD-53078 | ArrayExpress

Transcription profiling of wild type and DREB2C over-expression Arabidopsis plants

Project description:We analysed DREB2C overexpressing Arabidopsis plants (35S:DREB2C-a line: S15, 35S:DREB2C-c line: S22)with cDNA microarray (A-MEXP-187).

2007-03-10 | E-MEXP-986 | ArrayExpress

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and ir-lecRK1 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-lecRK1 plants. A total of 148 genes were identified as differentially regulated in ir-lecRK1 plants compared to wild type plants.

2011-11-23 | E-GEOD-29905 | ArrayExpress

Gene expression in aerial parts of rice-Arabidopsis Os-LBD37 FOX plants

Project description:Aerial parts of the rice-Arabidopsis FOX (Full-length cDNA overexpressor) lines K16331 and K19624 harboring the rice FL cDNA of LBD37 (Os-LBD37) were analyzed. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 ortholog of Arabidopsis in nitrogen metabolism- related processes. Overall design: K16331 (T2) and empty vector control plants (T2) were grown for 18 days on solid MS- medium supplemented with 20mg/l Hygromycin. For each hybridization on Affymetrix microarrays, 2 independent plants for K16331 were used. Homozygote K19624 (T3) and empty vector control plants (T3) were grown for 18 days on solid MS- medium. For each hybridization on Affymetrix microarrays, 1 plant was used.

2009-12-27 | GSE14646 | GEO

Expression data from 10-day old Arabidopsis seedlings treated with protease IV from Pseudomonas aeruginosa PA14

Project description:Immune responses in plants are triggered in part by conserved microbe-associated molecular pattern (MAMP) molecules such as bacterial flagellin. Upon MAMP perception, plants rapidly turn on the induction of numerous defense-related genes. We have identified a novel type of plant innate immunity elicitor, protease IV from Pseudomonas aeruginosa. Genome-wide transcriptomic profiles obtained with Affymetrix Arabidopsis ATH1 GeneChips® of 10-day old Arabidopsis seedlings treated with 20 nM purified protease IV for 1 hour were compared to published bacterial flagellin- and oligogalacturonide-triggered responses. We used microarrays to characterize the global transcriptomic changes in Arabidopsis seedlings upon protease IV treatment. Overall design: Ten-day old Arabidopsis seedlings were treated with mock control or 20 nM purified protease IV from Pseudomonas aeruginosa for 1 hour for RNA extraction and hybridization on Affymetrix microarrays.

| GSE58518 | GEO

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