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Dataset Information

36

HoxB4 in hematopoietic stem cells


ABSTRACT: Ectopic expression of homeodomain transcription factor HoxB4 in mouse ES cells enhances in vitro development of hematopoietic stem cells (HSCs). Overall design: Mouse ES cells were differentiated for 6 days in EBs, then c-Kit+CD41+ hematopoietic precursor cells were isolated, plated onto OP9 cells, and transduced with the GFP control or 3×FLAG-tagged HoxB4 retrovirus. At day 12 of the co-culture with OP9 cells, c-Kit+ progenitor cells overexpressing HoxB4 were collected by magnetic-activated cell sorting (MACS) and subjected to a ChIP-chip and microarray analysis. The purity of the recovered c-Kit+ cells was more than 95% as judged by flow cytometry.

INSTRUMENT(S): Agilent-014716 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 (G4490A) (Feature Number version)

ORGANISM(S): Mus musculus  

SUBMITTER: Takaho A. Endo  

PROVIDER: GSE26653 | GEO | 2011-05-23

SECONDARY ACCESSION(S): PRJNA136389

REPOSITORIES: GEO

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