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Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [scATAC-seq]

ABSTRACT: The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic reduandency, for cell fate decision of trophoblast cells. At stage of EPSC, inducible overexpression GATA2&3 reprogrammed hEPSCs to trophoblast lineage.

ORGANISM(S): Homo sapiens

PROVIDER: GSE266535 | GEO | 2026/09/01

REPOSITORIES: GEO

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Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [scRNA-seq]

Project description:The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic redundancy, for cell fate decision of trophoblast cells. At stage of EPSC, inducible overexpression GATA2&3 reprogrammed hEPSCs to trophoblast lineage. During differentiation of TSCM, double knockout GATA2&3 failed for initiation trophoblast cell fate.

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Project description:The GATA transcription factors, particularly GATA2 and GATA3 are responsible for trophoblast stem cells self renewal and differentiation in mice. However, we have a poor understanding about the contribution of GATA2 and GATA3, in the trophoblast cells in humans. Thus, the goal of this study is to define the importance of GATA factors in orchestrating global gene expression program in human trophoblast stem cells. Using a RNA interference strategy (RNAi), we depleted GATA2 or GATA3 expression in human trophoblast stem cells (GATA2 KD or GATA3 KD) . We then differentiated GATA2 KD and GATA3 KD human trophoblast stem cells into syncytiotrophoblasts(SynT) and compared global gene expression patterns via RNA sequencing (RNA-seq). Three individual experiments were performed for the differentiated SynT state. The submitted files contain the data (raw fastq files, count data and deseq2 output) from those experiments.

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Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [scATAC-seq]

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