Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [scRNA-seq]

ABSTRACT: The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic redundancy, for cell fate decision of trophoblast cells. At stage of EPSC, inducible overexpression GATA2&3 reprogrammed hEPSCs to trophoblast lineage. During differentiation of TSCM, double knockout GATA2&3 failed for initiation trophoblast cell fate.

ORGANISM(S): Homo sapiens

PROVIDER: GSE266536 | GEO | 2026/09/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [scATAC-seq]

Project description:The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic reduandency, for cell fate decision of trophoblast cells. At stage of EPSC, inducible overexpression GATA2&3 reprogrammed hEPSCs to trophoblast lineage.

2026-09-01 | GSE266535 | GEO

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [RNA-seq]

Project description:The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic redundancy, for cell fate decision of trophoblast cells. Using human expanded potential stem cells (hEPSC) as a model, we demonstrated that double knockout (DKO) of GATA2&3 failed for differentiation of trophoblast. Exogenous inducible over-expression GATA2&3 in DKO cells rescued trophoblast defects, in terms of gene expression.

2026-09-01 | GSE266534 | GEO

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [HiCUT]

Project description:The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic redundancy, for cell fate decision of trophoblast cells. Mechanistically, GATA2&3 maintain stemness of TSC by occupying open chromatin regions, mediating promoter activity, enhancer activity.

2026-09-01 | GSE278381 | GEO

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [ATAC-seq]

2026-09-01 | GSE266532 | GEO

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [CUT&Tag]

Project description:The commitment and differentiation of human placental progenitor cells are crucial for a successful pregnancy, but the underlying regulatory mechanism remains poorly understood. Here we identified the transcription factor (TF), GATA2 and GATA3, exerting genetic redundancy, for cell fate decision of trophoblast cells. Mechanistically, GATA2&3 maintain stemness of TSC by occupying open chromatin regions, mediating promoter activity, enhancer activity.

2026-09-01 | GSE266533 | GEO

RNA-Seq analysis in human trophoblast stem cells (CT27) upon depletion of transcription factor GATA2 or GATA3

Project description:The GATA transcription factors, particularly GATA2 and GATA3 are responsible for trophoblast stem cells self renewal and differentiation in mice. However, we have a poor understanding about the contribution of GATA2 and GATA3, in the trophoblast cells in humans. Thus, the goal of this study is to define the importance of GATA factors in orchestrating global gene expression program in human trophoblast stem cells. Using a RNA interference strategy (RNAi), we depleted GATA2 or GATA3 expression in human trophoblast stem cells (GATA2 KD or GATA3 KD) . We then differentiated GATA2 KD and GATA3 KD human trophoblast stem cells into syncytiotrophoblasts(SynT) and compared global gene expression patterns via RNA sequencing (RNA-seq). Three individual experiments were performed for the differentiated SynT state. The submitted files contain the data (raw fastq files, count data and deseq2 output) from those experiments.

2024-01-26 | GSE214634 | GEO

Cut and run analysis in human trophoblast stem cells (CT27) upon pulling down with GATA2 and GATA3 antibody

Project description:GATA transcription factors, particularly GATA2 and GATA3 are selectively expressed in the extraembryonic trophoblast lineage and regulates gene expression to promote trophoblast self-renewal during mammalian development. However, we have a poor understanding about the contribution of these GATA factors, in the process of syncytiotrophoblast (SynT) development. Thus, the goal of this study is to define the importance of GATA2 and GATA3 in orchestrating a global gene expression program that poises and commits mammalian trophoblast stem cells to the SynT lineage. Using the cut and run technique for GATA2 and GATA3 antibodies in the human trophoblast stem cells we are aiming to identify the direct targets of the GATA transcription factors that are essential for determining the SynT lineage. Three individual experiments were performed.

2024-01-26 | GSE214486 | GEO

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate.

Project description:Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate.

| PRJNA1107521 | ENA

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [HiCUT]

Project description:Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [HiCUT]

| PRJNA1166907 | ENA

Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [CUT&Tag]

Project description:Integrated multi-omic analysis of GATA2 and GATA3 unveil their master regulatory roles on the trophoblast fate [CUT&Tag]

| PRJNA1107531 | ENA