ChIP-Seq data for histone marks in mouse embryonic fibroblasts
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE26657 | GEO | 2011/01/19
SECONDARY ACCESSION(S): PRJNA136397
REPOSITORIES: GEO
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Project description:The conversion of mouse embryonic fibroblasts (MEFs) to induced pluripotent stem cells (iPS) by forced expression of Oct4, Sox2 and Klf4 is among the earliest demonstrations of reprogramming to a pluripotent state by forced expression of transcription factors. To gain insights into the chromatin state of genes required for reprogramming, we profiled H3K4me3, H3K27me3 and H3K9me3. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibody against H3K4me3, H3K27me3 and H3K9me3.
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