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Zebrafish 27hpf embryos: hdac1 mutant (hi1618) vs sibling

ABSTRACT: Transcriptional profiling of hdac1 mutant zebrafish in comparison to their sibling embryos. Embryos resulting from a cross between heterozygous hdac1 mutant zebrafish (hi1618/+) where cultured together then mutants separated from the siblings one the basis of phenotype and RNA extracted from the two groups at 27hpf was compared in a two-colour hybridisation.

ORGANISM(S): Danio rerio

PROVIDER: GSE26707 | GEO | 2011/01/21

SECONDARY ACCESSION(S): PRJNA142139

REPOSITORIES: GEO

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Zebrafish 27hpf embryos: hdac1 mutant (hi1618) vs sibling

Project description:Transcriptional profiling of hdac1 mutant zebrafish in comparison to their sibling embryos. Embryos resulting from a cross between heterozygous hdac1 mutant zebrafish (hi1618/+) where cultured together then mutants separated from the siblings one the basis of phenotype and RNA extracted from the two groups at 27hpf was compared in a two-colour hybridisation. Two-condition experiment, hdac1 mutants vs. sibling. Biological replicates: 2 (separate mating) Technical replicates: 4 (2 of which are dye-swap)

2011-01-21 | E-GEOD-26707 | biostudies-arrayexpress

Zebrafish embryos: hdac1 Morphants vs Standard control morphants

Project description:Transcriptional profiling of hdac1 morphant zebrafish embryos in comparison to standard control injected embryos. Embryos where injected at the one cell stage with either a translation blocking morpholino (Hdac1 ATG), splice blocking morpholino (Hdac1 Splice) or Hdac1 mismatch control morpholino (Hdac1 control). RNA was extracted and compared in a two-colour hybridisation to the comman reference Standard control morpholino injected embryos.

2011-01-21 | GSE26708 | GEO

Zebrafish hdac1 mutant and morphant embryos

Project description:This SuperSeries is composed of the following subset Series: GSE26707: Zebrafish 27hpf embryos: hdac1 mutant (hi1618) vs sibling GSE26708: Zebrafish embryos: hdac1 Morphants vs Standard control morphants GSE26709: Zebrafish embryos: hdac1 Morphants vs Standard control morphants at 12, 18 and 27 hpf Refer to individual Series

2011-01-21 | E-GEOD-26710 | biostudies-arrayexpress

Zebrafish embryos: hdac1 Morphants vs Standard control morphants at 12, 18 and 27 hpf

Project description:Transcriptional profiling of hdac1 ATG morphant zebrafish embryos in comparison to standard control injected embryos. Embryos where injected at the one cell stage with either a translation blocking morpholino (Hdac1 ATG) or Standard control morpholino (StCo). RNA was extracted at 12, 18 and 27hpf from both sets of embryos and compared with a two-colour hybridisation.

2011-01-21 | GSE26709 | GEO

Zebrafish embryos: hdac1 Morphants vs Standard control morphants

2011-01-21 | E-GEOD-26708 | biostudies-arrayexpress

Zebrafish embryos: hdac1 Morphants vs Standard control morphants at 12, 18 and 27 hpf

2011-01-21 | E-GEOD-26709 | biostudies-arrayexpress

Danio rerio

Project description:Zebrafish 27hpf embryos: hdac1 mutant (hi1618) vs sibling

| PRJNA142139 | ENA

Hai1 Mediates Inflammation Through MMP9

Project description:Adults heterozygous for the hi2217 retroviral insertion within the HAI locus (Mathias et al 2007 JCS) were in-crossed for RNA from hi2217 mutants and corresponding WT siblings. Adults heterozygous for the hi1520 retroviral insertion within the Clint1 locus (Dodd et al, 2009) were in-crossed for RNA from hi1520 mutants and corresponding WT siblings. Both hi2217 and hi1520 are mutants with epidermal defects and showing symptoms of chronic inflammation. In this study we aimed to compare the RNA expression changes between Wild-type siblings (a non-inflammatory status) and homozygous mutants (a chronic inflammatory status) for each mutant background (hi2217 and hi1520). Pools of 30-50 zebrafish embryos of WT siblings and hi2217 or hi1520 mutants were collected for RNA isolation. RNA from the hi2217 mutant embryos or hi1520 mutant embryos (test samples)was labelled with Cy5 and hybridized against Cy3-labelled RNA from the corresponding WT sibling embryos (reference samples) . These experiments were performed in biological triplicate.

2014-01-31 | E-GEOD-28110 | biostudies-arrayexpress

Comparsion of transcriptome between slbp1 mutant and wildtype sibling

Project description:The neurogenic state at transcriptomic level in delayed-neurogenesis zebrafish mutant "slbp1" with wildtype sibling was compared using bulk RNA sequencing data from RNA obtained from head regions at 2dpf.

2020-03-01 | GSE144517 | GEO

Project description:Zebrafish hdac1 mutant and morphant embryos

| PRJNA136353 | ENA

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