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Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

OTHER RELATED OMICS DATASETS IN: PRJNA142129PRJNA136327PRJNA142709

ORGANISM(S): Homo sapiens

PROVIDER: GSE26741 | GEO | 2011/06/02

SECONDARY ACCESSION(S): PRJNA136327

REPOSITORIES: GEO

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Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [expression]

Project description:Estrogen receptor α (ERα) is key player in the progression of breast cancer. ERα binds to DNA and mediates long-range chromatin interactions throughout the genome, but the underlying mechanism in this process is unclear. Here, we show that AP-2 motifs are highly enriched in the ERα binding sites (ERBS) identified from the recent ChIA-PET of ERα. More importantly, we demonstrate that AP-2γ (also known as TFAP2C), a member of the AP-2 family which has been implicated in breast cancer oncogenesis, is recruited to chromatin in a ligand-independent manner and co-localized with ERα binding events. Furthermore, pertubation of AP-2γ expression disrupts ERα DNA binding, long-range chromatin interactions, and gene transcription. Using ChIP-seq, we show that AP-2γ and ERα binding occurs in close proximity on a genome-wide scale. The majority of these shared genomic regions are also occupied by the pioneer factor, FoxA1. AP-2γ is required for efficient FoxA1 binding and vice versa. Finally, we show that most ERBS associated with long-range chromatin interactions are co-localized with both AP-2γ and FoxA1. Together, our results suggest AP-2γ is an essential factor in ERα-mediated transcription, primarily working together with FoxA1 to facilitate ERα binding and long-range chromatin interactions.

2011-06-02 | GSE26740 | GEO

Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [ChIP-Seq]

2011-06-03 | GSE23852 | GEO

Activator protein-2? is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [expression]

Project description: Not available

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Activator protein-2Î³ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [expression]

Project description:Estrogen receptor Î± (ERÎ±) is key player in the progression of breast cancer. ERÎ± binds to DNA and mediates long-range chromatin interactions throughout the genome, but the underlying mechanism in this process is unclear. Here, we show that AP-2 motifs are highly enriched in the ERÎ± binding sites (ERBS) identified from the recent ChIA-PET of ERÎ±. More importantly, we demonstrate that AP-2Î³ (also known as TFAP2C), a member of the AP-2 family which has been implicated in breast cancer oncogenesis, is recruited to chromatin in a ligand-independent manner and co-localized with ERÎ± binding events. Furthermore, pertubation of AP-2Î³ expression disrupts ERÎ± DNA binding, long-range chromatin interactions, and gene transcription. Using ChIP-seq, we show that AP-2Î³ and ERÎ± binding occurs in close proximity on a genome-wide scale. The majority of these shared genomic regions are also occupied by the pioneer factor, FoxA1. AP-2Î³ is required for efficient FoxA1 binding and vice versa. Finally, we show that most ERBS associated with long-range chromatin interactions are co-localized with both AP-2Î³ and FoxA1. Together, our results suggest AP-2Î³ is an essential factor in ERÎ±-mediated transcription, primarily working together with FoxA1 to facilitate ERÎ± binding and long-range chromatin interactions. Gene expression profiling of negative control (NC) and AP-2Î³ siRNA transfected MCF-7, with and without E2 (estradiol) stimulation using microarray.

2011-06-02 | E-GEOD-26740 | biostudies-arrayexpress

Activator protein-2M-NM-3 is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [ChIP-Seq]

Project description:Estrogen receptor M-NM-1 (ERM-NM-1) is key player in the progression of breast cancer. ERM-NM-1 binds to DNA and mediates long-range chromatin interactions throughout the genome, but the underlying mechanism in this process is unclear. Here, we show that AP-2 motifs are highly enriched in the ERM-NM-1 binding sites (ERBS) identified from the recent ChIA-PET of ERM-NM-1. More importantly, we demonstrate that AP-2M-NM-3 (also known as TFAP2C), a member of the AP-2 family which has been implicated in breast cancer oncogenesis, is recruited to chromatin in a ligand-independent manner and co-localized with ERM-NM-1 binding events. Furthermore, pertubation of AP-2M-NM-3 expression disrupts ERM-NM-1 DNA binding, long-range chromatin interactions, and gene transcription. Using ChIP-seq, we show that AP-2M-NM-3 and ERM-NM-1 binding occurs in close proximity on a genome-wide scale. The majority of these shared genomic regions are also occupied by the pioneer factor, FoxA1. AP-2M-NM-3 is required for efficient FoxA1 binding and vice versa. Finally, we show that most ERBS associated with long-range chromatin interactions are co-localized with both AP-2M-NM-3 and FoxA1. Together, our results suggest AP-2M-NM-3 is an essential factor in ERM-NM-1-mediated transcription, primarily working together with FoxA1 to facilitate ERM-NM-1 binding and long-range chromatin interactions. Genome-wide binding analysis of AP-2M-NM-3 and FoxA1 in MCF-7 with and without E2 (estradiol) stimulation using ChIP-Seq.

2011-06-03 | E-GEOD-23852 | biostudies-arrayexpress

Fucosyltransferase 8 regulation and breast cancer suppression by transcription factor activator protein 2γ.

Project description: Not available

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FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response

Project description:This SuperSeries is composed of the following subset Series: GSE25314: FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part I) GSE25315: FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part II) Refer to individual Series

2010-11-17 | E-GEOD-25316 | biostudies-arrayexpress

Project description: Not available

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FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response

Project description:This SuperSeries is composed of the SubSeries listed below.

2010-11-17 | GSE25316 | GEO

FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part I)

Project description: Not available

| S-ECPF-GEOD-25314 | biostudies-other