Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level from hippocampus of wild type mice and Camk2a-Vps13D KO mice

ABSTRACT: The failure to clear mitochondria, cell death and inflammation have been linked in neurodegenerative disease, but the relationship and role of inflammation in these conditions is not fully understood.To investigate it, we established a model for neurodegeneration caused by mitochondrial impairment by removing Vps13D from the hippocampus.We use single cell RNA sequencing to analyze the gene expression profile of wild type mice and Camk2a-Vps13D KO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE268343 | GEO | 2025/05/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression microarray analysis on the medial prefrontal cortex of Camk2a+/- mice and wild-type mice

Project description:The gene expression patterns in the medial prefrontal cortex of Camk2a+/- mice and wild-type mice were examined using Affymetrix GeneChip arrays.

2019-02-25 | GSE118724 | GEO

Camk2a-neuron specific FLAG-Ago HITS-CLIP

Project description:The HITS-CLIP procedure was used to detect miRNA mediated Argonaute (Ago) binding in Camk2a mouse neurons.

2023-01-31 | GSE211552 | GEO

Gene expression profile at single cell level from hippocampus of wild type mice and Camk2a-Vps13D KO mice

Project description:Gene expression profile at single cell level from hippocampus of wild type mice and Camk2a-Vps13D KO mice

| PRJNA1116244 | ENA

Aged vs Young Camk2a+ Neuron Proteome

Project description:Comparison of aged and young Camk2a+ neuronal proteome by transduction of mice with AAV-Camk2a;PheRS+ followed by AzF administration for BONCAT labeling of neuronal proteomes.

2025-11-07 | PXD057488 | Pride

Brain Region Camk2a Neuronal Protein Analaysis

Project description:Analysis of Camk2a+ neuronal proteome by brain region (hippocampus, striatum, cortex) by BONCAT labeling of Camk2a+ neurons, subsequent regional dissection, labeled-protein enrichment, and analysis by LC-MS.

2025-11-07 | PXD057261 | Pride

Abnormal microglia and enhanced inflammation-related gene transcription in mice with conditional deletion of Ctcf in Camk2a-Cre-expressing neurons [RPL_HC_CTCF]

Project description:CCCTC-binding factor (CTCF) is an 11 zinc finger DNA-binding domain protein that regulates gene expression by modifying 3D chromatin structure. Human mutations in CTCF cause intellectual disability and autistic features. Knocking out Ctcf in mouse embryonic neurons is lethal by neonatal age, but the effects of CTCF deficiency in postnatal neurons are less well studied. We knocked out Ctcf postnatally in glutamatergic forebrain neurons under the control of Camk2a-Cre. Ctcf loxP/loxP;Camk2a-Cre (Ctcf CKO) mice of both sexes were viable and exhibited profound deficits in spatial learning/memory, impaired motor coordination, and decreased sociability by 4 months of age. Ctcf CKO mice also had reduced dendritic spine density in the hippocampus and cerebral cortex. Microarray analysis of mRNA from Ctcf CKO mouse hippocampus identified increased transcription of inflammation-related genes linked to microglia. Separate microarray analysis of mRNA isolated specifically from Ctcf CKO mouse hippocampal neurons by ribosomal affinity purification identified upregulation of chemokine signaling genes, suggesting crosstalk between neurons and microglia in Ctcf CKO hippocampus. Finally, we found that microglia in Ctcf CKO mouse hippocampus had abnormal morphology by Sholl analysis and increased immunostaining for CD68, a marker of microglial activation. Our findings confirm that Ctcf KO in postnatal neurons causes a neurobehavioral phenotype in mice and provide novel evidence that CTCF depletion leads to overexpression of inflammation-related genes and microglial dysfunction.

2018-01-05 | GSE108826 | GEO

Abnormal microglia and enhanced inflammation-related gene transcription in mice with conditional deletion of Ctcf in Camk2a-Cre-expressing neurons [Whole_HC_CTCF]

2018-01-05 | GSE108825 | GEO

Camk2a-neuron specific FLAG-Ago HITS-CLIP

Project description:Camk2a-neuron specific FLAG-Ago HITS-CLIP

| PRJNA870889 | ENA

BONCAT Model Comparison in Camk2a+ Neurons

Project description:Comparison of three different BONCAT models (MetRS, PheRS, TyrRS) driven by Camk2a promoter to drive BONCAT machinery in excitatory neurons.

2025-11-07 | PXD057020 | Pride

Gene expression data from hippocampus, striatum, hypothalamus cortex, Drd2-MSNs and Drd1-MSNs in mice

Project description:Goal of the experiment: Analysis of gene expression changes in the cortex, striatum, hippocampus, hypothalamus, Drd2-MSNs and Drd1-MSNs of mice with a postnatal, neuron-specific ablation of GLP or G9a as compared to control mice. For microarray analysis, hippocampus, hypothalamus, cortex and striatum of Camk2a-Cre; GLPfl/fl, Camk2a-Cre; G9afl/fl and age (10-14 week old) and sex matched littermate controls were used for total RNA purification. Four biological replicates were performed for each experiment. Polyribosome associated mRNAs from five, age (10-14 week old) and sex matched Drd1-Cre; Drd1-bacTRAP; G9afl/fl, or Drd2-Cre; Drd2-bacTRAP; G9afl/fl and Drd1-bacTRAP; G9afl/fl or Drd2-bacTRAP; G9afl/fl control mice were used. Three biological replicates were performed for each experiment.

2009-12-10 | E-GEOD-19402 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data