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Small RNA sequencing reveals important miRNA function in Arabidopsis thaliana calli in response to combine drought and nitrogen deficiency

ABSTRACT: MiRNAs are important plant regulators responsible for growth, development and stress responses in plants.Previous studies have shown the roles of these miRNA on individual stress conditions, however their role in combined stress conditions such as nitrogen deficiency and drought are still unclear. Understanding the molecular mechanisms underlying plant responses to combined drought and nitrogen deficiency stress is essential for developing multiple stress-tolerant crops. In this study, Arabidopsis thaliana calli were subjected to combined drought and nitrogen deficiency, RNA extracted and then high throughput sequencing analysis. Analysis identified several differentially expressed miRNAs upregulated and downregulated. We also uncovered the target genes of these miRNAs. Gene ontology analysis provided insights into the different molecular, biological and cellular processes of these target genes. Overall, our findings contribute to the understanding of miRNA-mediated regulatory mechanisms under combined plant stress responses and offer valuable insights for the development of stress-tolerant crop varieties in stressors.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE268439 | GEO | 2026/05/29

REPOSITORIES: GEO

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