Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide distribution of REC8 cohesin is correlated with active transcription and regulated by transcription suppressor BEND2 during early meiosis [ATAC-seq]

ABSTRACT: To characterize REC8-binding sites on the genome in leptotene spermatocytes, we conducted ATAC-seq analyses by using leptotene spermatocytes isolated from wildtype mice through Hoechst 33342 staining and FACS. We found that REC8 preferentially binds to open promoter regions in leptotene spermatocytes.

ORGANISM(S): Mus musculus

PROVIDER: GSE268829 | GEO | 2026/06/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide distribution of REC8 cohesin is correlated with active transcription and regulated by transcription suppressor BEND2 during early meiosis [CUT&Tag]

Project description:To characterize REC8-binding sites on the genome, we conducted CUT&Tag analyses by using testicular cells of the Rec8-3×FLAG-2×HA knock-in (Rec8 KI) mice whose spermatogenesis was synchronized to preleptotene, leptotene or zygotene spermatocytes. We found that REC8 preferentially binds to open promoter regions of actively transcribed genes. Futhermore, we found the dynamic changes in REC8 binding sites during meiosis are correlated with gene transcriptional status. To describe the changes of REC8 binding sites in Bend2 knockout (Bend2 KO) mice, we conducted CUT&Tag analyses by using zygotene spermatocytes from Bend2 WT and KO mice in Rec8 KI background with HA antibodies. We found both up- and down-regulated REC8 peaks were observed despite of more down-regulated ones. Moreover, we found that these changed peaks were also located in open chromatin regions which was further increased in zygotene spermatocytes of Bend2 KO mice.

2026-06-30 | GSE268830 | GEO

Gene expression of pachytene-stage spermatocytes during male meiosis in Mus musculus [RNA-Seq]

Project description:Inhibition of pre‑rRNA transcription by BMH21 (12 h) in ex vivo mouse testicular single‑cell suspensions, followed by FACS isolation of pachytene spermatocytes (Hoechst 33342) and RNA‑seq. This dataset is designed to assess whether perturbing pre‑rRNA accumulation within the XY body affects the transcriptional landscape of pachytene spermatocytes, particularly regarding meiotic sex chromosome inactivation (MSCI). Comparison of BMH21‑treated vs control cells identifies differentially expressed autosomal and sex‑linked genes, providing insights into the role of nucleolar activity in sex chromosome regulation during male meiosis.

2026-05-28 | GSE328326 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [RNA-seq]

Project description:To describe the changes of transcriptome in Bend2 knockout mice, we conducted RNA-seq analyses by using leptotene and zygotene spermatocytes from Bend2 WT and KO mice. We found BEND2 contributes to shutting down the mitotic program and to activating or enhancing the meiotic and post-meiotic program of spermatogenic cells.

2022-06-01 | GSE189598 | GEO

Genome-wide binding of the meiosis specific Rec8 cohesin subunit in metaphase I

Project description:ChIP-seq profile of Rec8 binding sites arrested in metaphase I by depletion of the APC/C activator CDC20. The aim of this experiment is to identify Rec8 binding sites in metaphase I.

2019-04-25 | GSE123546 | GEO

DNA double-strand breaks regulate the cleavage-independent release of Rec8-cohesin during yeast meiosis

Project description:The mitotic cohesin complex necessary for sister chromatid cohesion and chromatin loop formation shows local and global association to chromosomes in response to DNA double-strand breaks (DSBs). Here, by genome-wide binding analysis of the meiotic cohesin with Rec8 as a kleisin, we found that Rec8 shows dynamic localization from middle to late meiotic prophase I with cleavage-independent global dissociation along chromosomes, driven by meiotic DSB formation. Each Rec8 binding site on the chromosome axis follows distinct dynamics with dissociation and association. Centromeres also showed reduced Rec8 binding in late prophase I relative to mid-prophase I, implying chromosome remodeling of the regions. Rec8 dissociation profile per chromosome is largely correlated with meiotic DSB density. Indeed, the spo11 mutant deficient in meiotic DSB formation did not show the cleavage-independent dissociation of Rec8 in late meiotic prophase I. These suggest the presence of a regulatory pathway for global Rec8-cohesin dynamics in response to meiotic DSBs.

2023-12-31 | GSE224074 | GEO

Screening of genes involved in chromosome segregation in meiosis I

Project description:Prophase I of male meiosis involves dynamic chromosome segregation processes during early spermatogenesis, including synapsis, meiotic recombination, and cohesion. Genetic defects in genes participating in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygotene spermatocytes, or pachytene spermatocytes were separately isolated from mouse testes for RNA extraction. To minimize the contamination of other cell types, we fractionated the testicular cells undergoing the first round of spermatogenesis using Percoll gradient procedure. The cell fractions were characterized by morphological analysis by phase contrast microscopy and Nomarski interference microscopy, and then expression of cell lineage- and spermatogenesis stage-specific genes were examined by RT-PCR. The most enriched fractions for spermatogonia (fraction2 from day8), leptotene/zygotene spermatocytes (fraction5 from day12), and pachytene spermatocytes (fraction8 from day15) were subjected to hybridization on Affymetrix microarrays.

2010-03-31 | E-GEOD-19532 | biostudies-arrayexpress

Genome-wide distribution of REC8 cohesin is correlated with active transcription and regulated by transcription suppressor BEND2 during early meiosis [ATAC-seq]

Project description:Genome-wide distribution of REC8 cohesin is correlated with active transcription and regulated by transcription suppressor BEND2 during early meiosis [ATAC-seq]

| PRJNA1118808 | ENA

Genome-wide distribution of REC8 cohesin is correlated with active transcription and regulated by transcription suppressor BEND2 during early meiosis [CUT&Tag]

Project description:Genome-wide distribution of REC8 cohesin is correlated with active transcription and regulated by transcription suppressor BEND2 during early meiosis [CUT&Tag]

| PRJNA1118813 | ENA

B-cell chronic lymphocytic leukemia side population cells

Project description:Analysis of Hoechst dye 33342-effluxing side population (SP) cells from B-CLL peripheral blood mononuclear cells.

2011-09-27 | GSE30171 | GEO

Genome-wide Map of R-loop Reveals its interplays with Transcription and Genome Integrity during Germ Cell Meiosis

Project description:R-loop is a three-strand nucleic acid structure composed of mRNA and DNA. In mammalian meiotic cells, R-loop mainly distributed at promoter-related regions, and combined with systematically transcriptome analysis showed R-loop was closely related to transcription in meiotic process. Moreover, knockout of Rnaseh1 in meiotic cells caused mice completely sterile with R-loop accumulation and abnormal DSB repairing in spermatocytes. Further analysis showed R-loop accumulation in leptotene/zygotene influenced transcriptional regulation in pachytene stage. Thus, mutual regulation of R-loop and transcription plays essential role in spermatogenesis.

2023-09-21 | GSE190430 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data