EV-mediated Monocytes Reprogramming in Mycobacterium tuberculosis Infection

ABSTRACT: Extracellular vesicles (EVs) secreted by Mtb-infected macrophages (MΦEVMtb) may influence the host innate immune response to Mtb-infection, but specific EV-induced mechanisms that regulate such cell phenotype changes are not yet known. We report that PMBCs cultured with MΦEVMtb exhibit a marked classical to intermediate monocyte polarization effect, as detected by single-cell RNA sequencing and flow cytometry results. Moreover, this effect appears to correspond with the activation of the alternative NF-κB pathway, and can be partially recapitulated by treating PBMCs with EV derived from recombinant cell line that overexpresses TRAF1, a direct regulator of this pathway. Collectively, we depict an EV-mediated monocyte reprogram landscape in the context of Mtb infection and highlight a potential link between EV and pathogenesis of tuberculosis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE269069 | GEO | 2026/06/30

REPOSITORIES: GEO