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RNA transcripts regulate G-quadruplex landscapes through G-loop formation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE269084 | GEO | 2025/04/02

REPOSITORIES: GEO

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RNA transcripts regulate G-quadruplex landscapes through G-loop formation

Project description:G-quadruplexes (G4s) are epigenetic DNA structures that regulate transcription but also threaten genome stability when dysregulated. It remains unknown how G4 dynamics is organized in a cell-specific manner. Here, we report that RNA transcripts govern G4 landscapes through homology-directed invasion opposite the G4 strand, forming ‘G-loops’. G-loops are disassembled via DHX36-FANCJ-mediated G4 unwinding and subsequent nucleolytic incision by XPF-ERCC1, leading to hybrid strand renewal through DNA synthesis. G-loop disassembly requires stochiometric invading RNAs, by contrast, excess of which stabilizes G-loops against disassembly. Defects in G-loop disassembly accumulate G-loops predominantly in gene regulatory regions, leading to transcriptional dysregulation, replication stress, and DNA damage throughout the cell cycle. These findings establish nuclear RNAs as pivotal G-loop regulators, linking the transcriptome to gene regulation and genome maintenance.

2025-06-20 | GSE268153 | GEO

RNA transcripts regulate G-quadruplex landscapes through G-loop formation (RNA-Seq)

Project description:G-quadruplexes (G4s) are prevalent DNA structures that regulate transcription but also threaten genome stability. How G4 dynamics is controlled remains poorly understood. Here, we report that RNA transcripts govern G4 landscapes through coordinated ‘G-loop’ assembly and disassembly. G-loop assembly involves activation of the ATM and ATR kinases followed by homology-directed invasion of RNA opposite the G4 strand mediated by BRCA2 and RAD51. Disassembly of the G-loop resolves the G4 structure via DHX36-FANCJ-mediated G4 unwinding that triggers nucleolytic incision and subsequent hybrid strand renewal by DNA synthesis. Inhibition of G-loop disassembly causes global G4 and R-loop accumulation, leading to transcriptome dysregulation, replication stress, and genome instability. These findings establish an intricate G-loop assembly-disassembly mechanism that controls G4 landscapes and is essential for cellular homeostasis and survival.

2025-04-02 | GSE269081 | GEO

RNA transcripts regulate G-quadruplex landscapes through G-loop formation (G4 and R-loop CUT&Tag)

2025-04-02 | GSE269082 | GEO

RNA transcripts regulate G-quadruplex landscapes through G-loop formation

Project description:RNA transcripts regulate G-quadruplex landscapes through G-loop formation

| PRJNA1114771 | ENA

RNA transcripts regulate G-quadruplex landscapes through G-loop formation

Project description:RNA transcripts regulate G-quadruplex landscapes through G-loop formation

| PRJNA1120071 | ENA

RNA transcripts regulate G-quadruplex landscapes through G-loop formation (RNA-Seq)

Project description:RNA transcripts regulate G-quadruplex landscapes through G-loop formation (RNA-Seq)

| PRJNA1120076 | ENA

RNA transcripts regulate G-quadruplex landscapes through G-loop formation (G4 and R-loop CUT&Tag)

Project description:RNA transcripts regulate G-quadruplex landscapes through G-loop formation (G4 and R-loop CUT&Tag)

| PRJNA1120075 | ENA

DNA 5-methylcytosine regulates genome-wide formation of G-quadruplex structures

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-20 | GSE221437 | GEO

Gcn5 and PCAF negatively regulate interferon β production through HAT-independent inhibition of TBK1

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Project description:This SuperSeries is composed of the SubSeries listed below.

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