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Cell cycle expression profiles in HeLa cells

ABSTRACT: Periodic expression of cell cycle genes highlights the importance of precise temporal control of transcription in regulating cell cycle events. We used HeLa cells enriched for different phases of the cell cycle to identify genes that are expressed in a periodic fashion in specific cell cycle phases. These data were generated for comparison with ChIP-Sequencing data obtained for two subunits of the B-Myb-MuvB complex, B-Myb and LIN9, in HeLa cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE26922 | GEO | 2012/01/25

SECONDARY ACCESSION(S): PRJNA142161

REPOSITORIES: GEO

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Cell cycle expression profiles in HeLa cells

Project description:Periodic expression of cell cycle genes highlights the importance of precise temporal control of transcription in regulating cell cycle events. We used HeLa cells enriched for different phases of the cell cycle to identify genes that are expressed in a periodic fashion in specific cell cycle phases. These data were generated for comparison with ChIP-Sequencing data obtained for two subunits of the B-Myb-MuvB complex, B-Myb and LIN9, in HeLa cells. HeLa cells were synchronized at the beginning of S phase by double thymidine block and then released into the cell cycle by washing out the thymidine resulting in tight synchrony at S, G2 and M phases of the cell cycle. Most cells entered mitosis at 8 hours after release as determined by visual inspection. Three independent replicas of double thymidine block and release experiments were performed. RNA was isolated at specific times after release from the thymidine block (0, 2, 4, 6, 8 and 12 hours) and used for generating expression profiles.

2012-01-25 | E-GEOD-26922 | biostudies-arrayexpress

Genome-wide binding profiles of the B-Myb-MuvB complex in HeLa cells

Project description:To identify genomic regions bound by B-Myb and LIN9 (a subunit of the MuvB complex), we performed ChIP-Sequencing (ChIP-Seq) using chromatin from proliferating HeLa cells in which we can detect a robust association between B-Myb and subunits of the MuvB complex. This analysis allowed us identify late cell cycle or G2/M expressed genes as specific targets of the B-Myb-MuvB complex. Examination of B-Myb and LIN9 binding in asynchronously growing HeLa cells

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The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression

Project description:This SuperSeries is composed of the following subset Series: GSE26922: Cell cycle expression profiles in HeLa cells GSE27030: Genome-wide binding profiles of the B-Myb-MuvB complex in HeLa cells Refer to individual Series

2012-01-25 | E-GEOD-27031 | biostudies-arrayexpress

Structure and molecular assembly of a MuvB transcription factor complex with the nucleosome

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