Dysregulation of cell state dynamics during early stages of serous endometrial carcinogenesis

ABSTRACT: Serous endometrial carcinoma (SEC) is one of the most aggressive and lethal types of uterine cancer, responsible for about 40% of all endometrial cancer-related deaths. Due to the rapid progression of SEC, early detection of this disease is of utmost importance. However, molecular and cellular dynamics during the pre-dysplastic stage of this disease remain largely unknown. Here, we provide a comprehensive census of cell types and their states for normal, pre-dysplastic, and dysplastic endometrium in a mouse model of SEC. This model is associated with inactivation of tumor suppressor genes Trp53 and Rb1, whose pathways are altered frequently in SEC. We report that pre-dysplastic changes are characterized by expanded and increasingly diverse immature luminal epithelial cell populations. Decrease of differentiated cell states is accompanied by a previously unreported reduction in number and strength of predicted interactions between epithelial and stromal endometrial cells. Such reduction is transient and is followed by formation of a new set of intercellular interactions marking further cancer progression. By using a multi-level approach combining single-cell and spatial transcriptomics paired with screening for clinically relevant genes in human endometrial carcinoma, we identified a panel of genes with potential use as early diagnostic, prognostic, and therapeutic targets. In summary, our results suggest an important role of luminal epithelial cell state in SEC pathogenesis, uncover transient reduction in cell-cell interactions prior the onset of cancer-associated dysplastic changes, and validate our mouse SEC model as a promising comparative platform in preclinical settings.

ORGANISM(S): Mus musculus

PROVIDER: GSE269332 | GEO | 2025/08/08

REPOSITORIES: GEO