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Effect of non-structural protein 1 (NS1) of H5N1 on CK2 expression, p53 signaling pathway and cell proliferation

ABSTRACT: NS1 (Non-structuralprotein1) is a non-structural protein of avian influenza viruses that can interact with a variety of host proteins. Our previous studies have demonstrated the interaction between NS1 and NOLC1 (nucleolus and coiled-coil phosphoprotein 1). To continue to investigate the mechanism of interaction, we constructed a cell line (A549-Flag-GFP-NS1) that stably expresses NS1. The expression of NS1, p53 and NOLC1 proteins was analysed by co-immunoprecipitation. The p53 signalling pathway in A549-Flag-GFP-NS1 cells was enriched for differentially expressed genes as analysed by transcriptome sequencing. qPCR experiments further verified that endogenous expression of NS1 altered the mRNA expression levels of p53 signalling-related genes (Bcl-2, CDK4, MDM2, cell cycle protein D, etc.). Meanwhile, endogenous expression of NS1 in A549 cells not only led to elevated expression of CK2, but also resulted in a significant increase in the phosphorylation levels of Ser46 and Ser392 as well as the acetylation level of Lys120 in p53 protein. The expression of relevant apoptotic factors in the p53 signalling pathway was further affected. These new findings provide new targets for pathogenic mechanism studies and drug screening.

ORGANISM(S): Homo sapiens

PROVIDER: GSE269479 | GEO | 2025/05/30

REPOSITORIES: GEO

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