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Screening the target genes of IRF10 in Japanese flounder, Paralichthys olivaceus

ABSTRACT: In previous study, we revealed that IRF10 is involved in the immune-reponse against bacteria and virus in Paralichthys olivaceus. However, the target genes of IRF10 is still unknown in teleosts. We searched the target genes of IRF10 by microarray in IRF10 over expressed HINAE cell

ORGANISM(S): Paralichthys olivaceus

PROVIDER: GSE26959 | GEO | 2015/03/01

SECONDARY ACCESSION(S): PRJNA136969

REPOSITORIES: GEO

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Screening the target genes of IRF10 in Japanese flounder, Paralichthys olivaceus

Project description:In previous study, we revealed that IRF10 is involved in the immune-reponse against bacteria and virus in Paralichthys olivaceus. However, the target genes of IRF10 is still unknown in teleosts. We searched the target genes of IRF10 by microarray in IRF10 over expressed HINAE cell HINAE cells were culured in 24-well plate. HINAE cells were transfected using lipofectamine2000 (Invitrogen, USA) with 7.2nM IRF10 expression plasmid vector (pcDNA3.1-IRF10) or empty-vector (pcDNA3.1). The equal volume of sterile water was used as a control. On other hands, the 1μg/ml of poly I:C also transfected to HINAE cells using lipofectamine2000 with each plasmid and vehicles (sterile water). After 48 hours from the transfection, we harvested the two samples of the control and poly I:C only and the three samples of plasmids or plasmids with poly I:C transfected cells (16 hybridization).

2015-03-01 | E-GEOD-26959 | biostudies-arrayexpress

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Transcriptome data involved in Japanese flounder (Paralichthys olivaceus) albinism

Project description:In this study, a high-throughput sequencing strategy was employed to identify the mRNA involved in P. olivaceus albinism. Based on P. olivaceus genome, RNA-seq identified 21,787 know genes and 711 new genes by transcripts assembly. Of those, 235 genes exhibited significantly different expression pattern (fold change ≥2 or ≤0.5 and q-value≤0.05), including 194 down-regulated genes and 41 up-regulated genes in albino versus normally pigmented individuals. These genes were enriched to 81 GO terms and 9 KEGG pathways (p≤0.05). Among those, the pigmentation related pathways-Melanogenesis and tyrosine metabolism were contained.

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Project description:Paralichthys olivaceus Heart Transcriptome under low temperature stress

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Paralichthys olivaceus breed:Japanese flounder

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Paralichthys olivaceus breed:Japanese flounder

Project description:Paralichthys olivaceus breed:Japanese flounder Raw sequence reads

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microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

Project description:In this study, a high-throughput sequencing strategy was employed to identify the miRNAs involved in P. olivaceus albinism. High-throughput miRNA sequencing identified a total of 475 miRNAs, including 64 novel miRNAs. Furthermore, 33 differentially expressed miRNAs containing 13 up-regulated and 20 down-regulated miRNAs were identified in albino versus normally pigmented individuals (fold change ≥1.5 or ≤0.67 and p≤0.05).

2017-07-13 | GSE99342 | GEO

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