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Bj H2O2 prolonged exposure vs. non-treatment

ABSTRACT: Genome-wide transcriptional profiling of cells exposed to 0.3 mM H2O2 from time of inoculation.

ORGANISM(S): Bradyrhizobium japonicum Bradyrhizobium diazoefficiens USDA 110

PROVIDER: GSE26960 | GEO | 2011/07/30

SECONDARY ACCESSION(S): PRJNA136971

REPOSITORIES: GEO

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Bj H2O2 prolonged exposure vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells exposed to 0.3 mM H2O2 from time of inoculation. Three independent biological materials were prepared for cells exposed to 0.3 mM H2O2 during growth and non-treated cells. A total 6 arrays which includes dye swap were analyzed

2011-07-29 | E-GEOD-26960 | biostudies-arrayexpress

Bj Paraquat prolonged exposure vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells exposed to 0.1 mM paraquat from time of inoculation.

2011-05-16 | GSE26252 | GEO

Bj H2O2 shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes).

2011-07-30 | GSE26961 | GEO

Bj Paraquat prolonged exposure vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells exposed to 0.1 mM paraquat from time of inoculation. Three independent biological materials were prepared for cells exposed to 0.1 mM paraquat during growth and non-treated cells. A total 6 arrays which includes dye swap were analyzed.

2011-05-15 | E-GEOD-26252 | biostudies-arrayexpress

Bj H2O2 shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes). Three independent biological materials were prepared for cells shock treated with H2O2 and non-treated cells. A total 6 arrays which includes dye swap were analyzed.

2011-07-29 | E-GEOD-26961 | biostudies-arrayexpress

A transcriptome comparison between pao5 vs NIP, ACC-treatment vs Mock (NIP) and H2O2-treatment vs Mock (NIP)

Project description:OsPAO5 mainly functions to oxidize spermine and produce H2O2,which finally affects mesocotyl elongation in rice. Meanwhile ethylene and polyamines synthesis crosstalk has been reported in competing the common substrate. To further confirm the relationship between H2O2 and ethylene in contributing to the mesocotyl length, we conducted a transcriptome comparison between pao5-1 vs NIP, ACC-treatment vs Mock (NIP) and H2O2-treatment vs Mock (NIP).

2023-06-26 | GSE153423 | GEO

H2O2 Treatment of Human PBMC

Project description:To mimic the effect of ROS in contributing to ageing process in immune cells of PBMC. The PBMC cells were harvested from old human subjects (>50) and induced with H2O2 during 3 hour and 6 hour. The CEL file were normalized using RMA methods. We used microarray to explore the gene expression profiling before and after ROS. Gene related to cell cycle, apoptosis, metabolism and signal transduction were differentially expressed after inducing with H2O2 in compared to baseline.

2010-06-20 | GSE22463 | GEO

Identification of putative H2O2 regulated genes during the establishment of the Sinorhizobi...

Project description:The involvement of ROS in the legume – Rhizobium symbiotic interaction has been highlighted (Santos et al., 2001; Rubio et al., 2004). This interaction is characterized by the formation of a new organ on the root, the nodule and by the penetration, in parallel, of the bacteria into the root tissue via an infection thread (IT) (Parniske and Downie, 2003; Gage, 2004). H2O2 production has been shown in ITs during the Medicago – Sinorhizobium meliloti interaction (Santos et al., 2001). Moreover, S. meliloti mutants impaired in H2O2 detoxification mechanism possess in planta symbiotic phenotypes. As H2O2 production is known to orchestrate plant gene expression, our goal is focused on identifying the H2O2 regulated genes in the symbiotic process. We focus our analysis in the M. truncatula transcriptome as we are characterising the S. meliloti H2O2 transcriptome. -M. truncatula seedlings were grown in axenic condition on modified Fahraeus medium during seven days. Then they were transferred on new plates supplemented either with dimethyl sulfoxide (DMSO, mock treatement) or diphenylene iodonium (DPI, 10 µM). Twenty four hours later, they were inoculated either with water (mock inoculation; DMSO-H2O and DPI-H2O) or wild type S. meliloti 2011 strain (DMSO-INOC and DPI-INOC). Roots without apexes were harvested 48 hours after inoculation. After RNA extraction (Trizol), quality of the treatment was verified by RT-PCR (ENOD11: inoculation efficiency; GSHS1: DNA contamination; MTC27: constitutive) Keywords: treated vs untreated comparison

2010-01-01 | GSE15866 | GEO

H2O2 Treatment of Human PBMC

2010-07-08 | E-GEOD-22463 | biostudies-arrayexpress

Gene expression in response to chloroplastic H2O2: IS-GUS-2-17 vs. IS-tAPX-19-23 under estrogen treatment

Project description:To identify chloroplastic H2O2-responseive genes, an estrogen-inducible RNAi method was used for silencing of tAPX, a H2O2-scavenging enzyme in chloroplasts. At 48 h after treatment with estrogen, the expression of tAPX was silenced in the IS-tAPX-19-23 plants, but not in the IS-GUS-2-17 plants (negative control), resulting a large change in gene expression. These genes are candidates for chloroplastic H2O2-responseive genes.

2012-02-04 | GSE35526 | GEO

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