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Bj H2O2 shock vs. non-treatment

ABSTRACT: Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes).

ORGANISM(S): Bradyrhizobium diazoefficiens USDA 110 Bradyrhizobium japonicum

PROVIDER: GSE26961 | GEO | 2011/07/30

SECONDARY ACCESSION(S): PRJNA136973

REPOSITORIES: GEO

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Bj H2O2 shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes). Three independent biological materials were prepared for cells shock treated with H2O2 and non-treated cells. A total 6 arrays which includes dye swap were analyzed.

2011-07-29 | E-GEOD-26961 | biostudies-arrayexpress

Bj Paraquat fulminant shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a paraquat fulminant shock treatment (5mM for 10 minutes).

2011-05-16 | GSE26236 | GEO

Bj Paraquat fulminant shock vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells subjected to a paraquat fulminant shock treatment (5mM for 10 minutes). Three independent biological materials were prepared for cells shock treated with paraquat and non-treated cells. A total 6 arrays which includes dye swap were analyzed.

2011-05-15 | E-GEOD-26236 | biostudies-arrayexpress

Bj H2O2 prolonged exposure vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells exposed to 0.3 mM H2O2 from time of inoculation.

2011-07-30 | GSE26960 | GEO

Bj H2O2 prolonged exposure vs. non-treatment

Project description:Genome-wide transcriptional profiling of cells exposed to 0.3 mM H2O2 from time of inoculation. Three independent biological materials were prepared for cells exposed to 0.3 mM H2O2 during growth and non-treated cells. A total 6 arrays which includes dye swap were analyzed

2011-07-29 | E-GEOD-26960 | biostudies-arrayexpress

Halobacterium NRC-1, H2O2 Stress Response

Project description:50mM H2O2 was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of H2O2 for 30 minutes, cultures were spun down and pellets were resuspended in same volume of GN101 media. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes. Keywords: stress response

2008-01-03 | GSE7610 | GEO

Halobacterium NRC-1, H2O2 Stress Response

2010-05-27 | E-GEOD-7610 | biostudies-arrayexpress

Cells pretreated with NaCl have an altered gene expression in response to H2O2.

Project description:BY4741 (S288c haploid) cells have different gene expression in response to 0.4mM H2O2 when pretreated with 0.7M NaCl compared to cells that are not pretreated with NaCl (mock cells). BY4741 cells were grown in log phase for 3 doublings (t0) and then the genomic expression of mock cells to 0.4mM H2O2 was assessed every 10 mins for 40 mins. To study the effects of NaCl treatement on H2O2 response, BY4741 cells were grown in log phase for 3 doublings (t0) and then the genomic expression of cells was measured in response to 0.7M NaCl over the course of 60 min every 15 mins. The cells were then removed from stress and grown in stress free media for four hours (T240). Then these cells were exposed to 0.4mM H2O2 and the genomic expression was measured every 10 minutes during the H2O2 timecourse of 40 minutes. To see if the handling of cells had an affect on gene expression, BY4741 cells were grown for exponentially for 3 doublings (t0), received a mock YPD treatment for 60 mins, grown for 4 hours (T240) and then collected to asses genomic expression. Duplicates were done for at 30 and 45 minutes for the NaCl timecourse, triplicate were done for 0, 10 and 20 minutes in H2O2 timecourse and duplicates were done for 30 and 40 minutes in the H2O2 timecourse.

2011-09-17 | E-GEOD-32196 | biostudies-arrayexpress

Transcriptome analysis of Arabidopsis thaliana roots, H2O2 and Rose Bengal [20180920_173313_H2O2_RB]

Project description:In order to better understand the context of root water stress and its relationship to other stresses, I undertook an evaluation of osmotic water stress by transcriptome analyses. Whole transcriptome MARSeq (Diego Adhemar Jaitin, 2017) analysis can enable us to study the changes in gene expression (changes in transcript prevalence) in depth under different treatments. It is a cost effective method to develop transcriptional activity. It uses relatively small amounts of RNA and reads sequences from the 3’-polyA containing end. This method can reveal underlying trends that can help us understand the less obvious processes happening in our system. To carry this out I processed whole transcriptome data separately from WT seedling roots exposed to 5 mM H2O2 or 10 µM RB for 0, 1, 2, 3 h or 5 mM H2O2 or 10 µM for 2h .

2021-05-26 | GSE152455 | GEO

Transcriptome analysis of Arabidopsis thaliana roots, H2O2 and Rose Bengal [20180920_172844_1O_H2O2]

2021-05-26 | GSE152454 | GEO

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