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Inner-envelope phosphatidylethanolamine promotes DNA replication and nucleoid- biogenesis in early chloroplast development

ABSTRACT: Proplastid-to-chloroplast transitconversion during early plant development involves exintensive genome replication and nucleoid structurale changes of the nucleoid during plant early development. In addition, and the nucleoid distribution shifts from ring-shaped assemblies near the inner- envelope to thylakoids-anchored punctate structures., while the regulatoryThe mechanisms underlying this massive reorganization are still missingnot known. Here we report that the glycerophospholipid phosphatidylethanolamine (PE) governs early chloroplast genome replication and nucleoid biogenesis. Genetic screens revealed that Through genetic screening, we found mutations ofin the phosphatidylserine decarboxylase PSD1 can relieve the transcription-replication conflicts (TRCs) in thea chloroplast R-loop accumulation mutant atrnh1c. PSD1 is thea chloroplast inner- envelope-localized protein that required for PE synthesis. PE reduction leads to decreased replication speed and TRCs stresses, resulting in fewer less R-loops and DNA breaks. We also show that PE physically interacts with replication-related proteins and nucleic acids, promotes DNA replication and nucleoid concentration in the inner- envelope during early seedling development, accomplishing proplastid-to-chloroplast transition. Together, our results reveal a previously unknowndiscovered lipid-involvbased mechanism for genome maintenance and nucleoid- biogenesis, and open up a new horizonsuggest a role for lipids in participating genomeome regulation events.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE270028 | GEO | 2026/05/22

REPOSITORIES: GEO

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