Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide maps of chromatin state in rice endosperm

ABSTRACT: We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone H3 trimethylation in rice endosperm. By obtaining about four hundred million bases of sequence from rice chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of rice endosperm. We find that the presence of H3K27me3 in either upstream or downstream of a gene is predominately associated with repression of the gene, while its absence is mainly associated with high gene expression.

ORGANISM(S): Oryza sativa

PROVIDER: GSE27048 | GEO | 2011/12/06

SECONDARY ACCESSION(S): PRJNA141937

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide maps of chromatin state in rice endosperm

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone H3 trimethylation in rice endosperm. By obtaining about four hundred million bases of sequence from rice chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of rice endosperm. We find that the presence of H3K27me3 in either upstream or downstream of a gene is predominately associated with repression of the gene, while its absence is mainly associated with high gene expression. Examination of Histone H3 lysine 27 trimethylation in rice endosperm.

2011-12-06 | E-GEOD-27048 | biostudies-arrayexpress

Rice endosperm: mRNA profiling and H3K27me3 occupancy

Project description:This SuperSeries is composed of the following subset Series: GSE26840: Express data from rice endosperm GSE27048: Genome-wide maps of chromatin state in rice endosperm Refer to individual Series

2011-12-06 | E-GEOD-27064 | biostudies-arrayexpress

Genome-wide maps of H3K27 state in transfected NIH3T3 cells.

Project description:Epigenetic H3k27me3 changes of mouse NIH3T3 cells comparing control NIH3T3 cells transfected with a pEFm6-BRAF with cells transfected with pEFm6-BRAFV600E. By obtaining over 30 Million bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of BRAF transfected. We find that global lysine 3 and lysine 27 trimethylation levels changed under the condition of activated ERK by BRAFV600E, and specific genes might be downregulated for the hight level of H3K27me3 induced by BRAFV600E

2017-01-01 | GSE93033 | GEO

Developing rice endosperm at seven days after flowering: Aleurone layers vs central starchy endosperm

Project description:Transcriptional profiling of developing rice endosperm at seven days after flowering comparing aleurone layers with central starchy endosperm. Cereal productivity is dependent on the accumulation of storage compounds in the endosperm, a nutritive tissue that is composed of aleurone cells in the outermost regions and starchy endosperm in the inner region. The transcriptional analyses provides clues to the molecular basis for different metabolic pathways in response to the spatial and nutritional differences between rice aleurone cells and starchy endosperm.

2012-11-27 | GSE42489 | GEO

Genome-wide maps of H3K4me3 and H3K27me3 in HY(Hwayong) and chd1(chr729) rice.

Project description:We report the application of CHIP-seq technology for high-throughput profiling of histone modifications in HY(Hwayong) and chd1(chr729). By obtaining about 12 million reads of sequence from chromatin immunoprecipitated DNA, we generated genome-wide H3K4me3 and H3k27me3 maps of HY(Hwayong) and chd1(chr729). We find that lysine 4 and lysine 27 trimethylation marked genes effectively losed in chd1 compared to HY. Examination of two histone modifications in two rice species.

2012-03-10 | E-GEOD-30490 | biostudies-arrayexpress

KANSL1 CHIP-sequence analysis in HeLa cells

Project description:We report the application of CHIP-sequence in HeLa cells. By obtaining over fifty million bases of sequence from chromatin immunoprecipitated DNA for each sample, we generated genome-wide chromatin-state maps of HeLa cells.

2020-10-13 | GSE159386 | GEO

KANSL1 ChIP-sequence analysis in HeLa cells

Project description:We report the application of ChIP-sequence in HeLa cells. By obtaining over fifty million bases of sequence from chromatin immunoprecipitated DNA for each sample, we generated genome-wide chromatin-state maps of HeLa cells.

2021-06-10 | GSE176425 | GEO

Genome-wide Runx1 binding sites in early hematopoietic progenitors (FDCP1)

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 36 trimethylation marks primary coding and non-coding transcripts, facilitating gene annotation. Trimethylation of lysine 9 and lysine 20 is detected at satellite, telomeric and active long-terminal repeats, and can spread into proximal unique sequences. Lysine 4 and lysine 9 trimethylation marks imprinting control regions. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations. ChIP analysis of Runx1 binding sites in early hematopoietic progenitors (FDCP1 and FDCP1_RUNX1-ERt2)

2016-07-01 | E-GEOD-81179 | biostudies-arrayexpress

Genome-wide maps of Set1 and H3K4me3 in budding yeast

Project description:We report the application of ChIP-sequencing technology for high-throughput profiling of histone modifications in budding yeast. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide maps of Set1 and H3K4me3 in yeast cells. We find that Set1 and H3 lysine 4 trimethylation locate primarily in open reading frame of genes. In addition, we focus on the distribution of Set1 and H3K4me3 in histone gene clusters and found strong similar binding of Set1 and H3K4me3 on all histone genes.

2019-05-02 | GSE115910 | GEO

Genome-wide maps of chromatin state in liver cells.

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse liver cells. We find that lysine 4 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 9 and 27 trimethylation marks primary coding and non-coding transcripts, have no differents in gene annotation. Trimethylation of lysine 4 is detected has different expresssion at mir124 site front of lnc-RNA(10835)Far. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations.

2020-03-08 | GSE128073 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data