Transcriptomics

Dataset Information

30

Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum


ABSTRACT: Comparison of the gene expression profile of Moraxella catarrhalis grown in the presence of 20% pooled human sputum in chemically-defined medium relative to Moraxella catarrhalis grown in chemically-defined medium alone. Overall design: Moraxella catarrhalis ATCC43617 was grown to mid-logarithmic phase either in the presence of 20% pooled human sputum in chemically-defined medium or in chemically-defined medium alone. Total RNA was extracted from bacterial cells exposed to each of these conditions and cDNA was generated for CyDye labelling. 3 biologic replicates were generated and each replicate underwent a dye swap (total of 6 experimental data collections). The gene expression profile reported is that of Moraxella catarrhalis grown in the presence of pooled human sputum in a chemically-defined medium relative to Moraxella catarrhalis grown only in the presence of the chemically-defined medium.

INSTRUMENT(S): EJH Mcat Oligo-DNA Microarray 3

ORGANISM(S): Moraxella catarrhalis ATCC 43617  

SUBMITTER: Todd C. Hoopman  

PROVIDER: GSE27059 | GEO |

SECONDARY ACCESSION(S): PRJNA137619

REPOSITORIES: GEO

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