Dataset Information

Tertiary lymphoid structures at varied stages exhibit distinct anti-tumorigenic immunity [Spatial Transcriptomics]

ABSTRACT: The tumour microenvironment (TME) is a focal point in cancer immunotherapy: its cellular composition and spatial organisation, especially the distribution of lymphocytes, can affect the clinical outcomes of cancer patients. In addition, the function of a cell differs depending on its spatial location and interaction with neighbouring cells. Here, by integrating single-cell transcriptomics with spatial transcriptomics, we survey how the spatial distribution of different cell types varies across diverse histological regions of gastric cancer. Notably, tertiary lymphoid structures (TLSs), a tissue architecture harbouring lymphocytes more than any other region within the TME, possess great potential in modulating anti-tumorigenic immunity by elevating an array of genes (FDCSP and CCL19) principally involved in lymphocyte recruitment and activation. Our findings advance the understanding of TLSs in contributing to anti-tumorigenic immunity in a spatially resolved context, which could be further leveraged as a predictive marker for immunotherapy response.

ORGANISM(S): Homo sapiens

PROVIDER: GSE270678 | GEO | 2025/12/03

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Background and aims: Tertiary lymphoid structures (TLSs) are formed in many cancer types and have been correlated with better prognosis and response to immunotherapy. In liver cancer, TLSs have been reported to be pro-tumorigenic as they harbor tumor progenitor cells and nurture their growth. The processes involved in TLS development and the acquisition of a pro- or anti-tumorigenic phenotype in cancer are largely unknown. RORc expressing immune cells have been previously implicated in TLS formation, however, their role in TLS development in the context of inflammation-associated liver cancer remains unexplored. Methods: IKKβ(EE)Hep mice, exhibiting chronic liver inflammation and TLS formation followed by liver cancer, were crossed with RORc knockout mice to explore the effect of RORc on TLS and tumor formation. Transcriptional, proteomic and immunohistochemical techniques were used to analyze TLS phenotypes. CD4, CD8 and B cell depletions were used to analyze their contribution to liver TLS and tumor formation. Results: RORc expressing cells were detected within TLSs of both human patients and mice which develop intrahepatic cholangiocarcinoma. In mice, RORc expressing cells negatively regulate TLS formation, since in their absence TLSs form in excess. CD4 cells are essential for liver TLS formation whereas B cells are required for TLS formation specifically in the absence of RORc expressing cells. Importantly, in chronically inflamed livers lacking RORc expressing cells, TLSs become anti-tumorigenic, resulting in reduced tumor load. Comparing liver pro- and anti-tumorigenic TLSs, revealed enrichment of exhausted CD8 cells that retained effector functions, as well as germinal center B cells and plasma cells in anti-tumorigenic TLSs. Cell depletion experiments revealed a role mainly for B cells in limiting tumor development, possibly via tumor-directed antibodies. Conclusions: RORc expressing cells negatively regulate B cell responses, and facilitate the pro-tumorigenic functions of hepatic TLSs.

Dataset Information

Tertiary lymphoid structures at varied stages exhibit distinct anti-tumorigenic immunity [Spatial Transcriptomics]

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets