Inactivation of the conserved protease LonA increases production of xylanase and amylase in Bacillus subtilis

ABSTRACT: In a previous study we used RNA-seq to identify cellular stresses related to the overexpression of xylanase XynA, and found that upregulation of the CtsR regulon improves the yield of XynA production in B. subtilis. In this study, we compared the transcriptomes of B. subtilis cells overexpressing either the xylanase XynA or the heterologous amylase AmyM, to identify general and enzyme-specific stress responses. In addition, we further compared the translational profiles of cells overexpressing XynA and AmyM using ribosome profiling.

ORGANISM(S): Bacillus subtilis

PROVIDER: GSE270692 | GEO | 2024/08/30

REPOSITORIES: GEO