Longitudinal scRNA-seq analysis of human liver bud organoid (HLBO) with iPS-derived liver sinusoidal endothelial progenitor (iLSEP)

ABSTRACT: We directly differentiated human pluripotent stem cells into CD32b+ putative liver sinusoidal progenitors (iLSEP) by dictating developmental pathways. By devising an inverted multilayered air-liquid interface (IMALI) culture, hepatic endoderm, septum mesenchyme, arterial and sinusoidal quadruple progenitors self-organized to generate vascularized liver bud organoid. To uncover cellular characteristics and diversity during organoid development at transcriptomic level, time-series single cell RNA-seq (scRNA-seq) data were acquired.

ORGANISM(S): Homo sapiens

PROVIDER: GSE270807 | GEO | 2024/08/02

REPOSITORIES: GEO