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Changes in gene expression of Pinus armandii seed kernels during the harvesting period (August to September)

ABSTRACT: The illumina NovaSeq6000 platform was utilized to generate mRNA profiles of Pinus armandii seednuts obtained in August and September. Total RNA was extracted from seed kernels from each month separately and pooled into three biological replicates for each condition. The concentration and purity of RNA (OD260/280 and OD260/230 ratios) were measured using a NanoPhotometer spectrophotometer. RNA concentration was examined for accurate quantification using a Qubit 2.0 Fluorometer, and then RNA integrity was accurately examined using an Agilent 2100 bioanalyzer.

ORGANISM(S): Pinus armandii

PROVIDER: GSE271040 | GEO | 2025/07/31

REPOSITORIES: GEO

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Project description:Fusarium verticillioides is a detrimental fungus that can contaminate maize grains with mycotoxins that are harmful to human and animal health. Breeding and growing resistant genotypes is one alternative to reduce contamination and subsequent production of mycotoxins by this fungus. However, little is known about the resistant mechanism relevant to breeding in this pathosystem. Therefore, our aim was to identify genes and metabolites that may be related to Fusarium ear rot resistance using resistant and susceptible maize inbreds. Kernels of the resistant inbred showed significantly reduced disease severity, and reduced levels of total fumonisin and ergosterol content compared with the susceptible one. Gene expression data were obtained from microarray hybridizations using F. verticillioides inoculated and non inoculated maize kernels. Differentially expressed sequences were identified and classified into 36 functional categories. Most of the differentially expressed genes were assigned to the categories “protein, RNA, DNA, stress, transport, signaling and cell metabolism”. These genes encode for PR proteins, detoxification and primary metabolism enzymes. Fungal inoculation did not produce considerable changes in gene expression and metabolites in the resistant L4637 inbred, probably due to a preformed or constitutive resistance mechanism. Defense-related genes were induced or repressed in kernels of the susceptible inbred L4674, responding specifically to the pathogen infection. The qRT-PCR in infected silks showed that glucanase, lipid transfer, xylanase inhibitor, PR1 and 26S proteosome transcripts had higher expression ratios in the susceptible line compared to the resistant one in response to fungal infection. Through this study, a global view of differential genes expressed and metabolites concentration during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.

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Changes in gene expression of Pinus armandii seed kernels during the harvesting period (August to September)

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