TnSeq study evaluating the impact of Atg5 and Atg7 on survivial of Mycobacterium tuberculosis transposon mutants in IFN-gamma-activated murine bone marrow-derived macrophages

ABSTRACT: Mycobacterium tuberculosis impairs host lysosomal trafficking pathways. Xenophagy and LC3-associated phagocytosis (LAP) are lysosomal trafficking pathways that normally clear intracellular microbes. The xenophagy and LAP pathways depend upon ATG5 and ATG7, which result in the lipidation of LC3-I to LC3-II. How Mtb undermines these innate immune defenses of the host in not well understood. We used a transposon sequencing (Tn-seq) screen to identify bacterial factors that are required for the bacilli to resist ATG5 and ATG7-dependent processes. We found that that mutants defective in production of PDIM are attenuated in murine macrophages, and they are able to survive in macrophages lacking ATG5 or ATG7.

ORGANISM(S): Mycobacterium tuberculosis

PROVIDER: GSE271206 | GEO | 2024/07/08

REPOSITORIES: GEO