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The role of CBLL1 (HAKAI) component of the RNA methyltransferase complex in basal and estrogen signaling in breast cancer cells (MCF-7)

ABSTRACT: CBLL1, also known as HAKAI, plays a dual role in the METTL3-containing RNA m6A methyltransferase complex and as a regulator of e-cadherin protein expression. RNA methylation is known to play diverse functions in the regulation of mRNA processing, splicing, translation and stability. In this study we used siRNA to deplete CBLL1 in the MCF-7 breast cancer cell line and determined the effect on basal and estrogen regulated transcription

ORGANISM(S): Homo sapiens

PROVIDER: GSE271326 | GEO | 2025/11/30

REPOSITORIES: GEO

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The role of CBLL1 (HAKAI) component of the RNA methyltransferase complex in androgen signaling in breast cancer cells (MDA-MB-231)

Project description:CBLL1, also known as HAKAI, plays a dual role in the METTL3-containing RNA m6A methyltransferase complex and as a regulator of e-cadherin protein expression. RNA methylation is known to play diverse functions in the regulation of mRNA processing, splicing, translation and stability. In this study we used siRNA to deplete CBLL1 in the MDA-MB-231 triple negative breast cancer cell line and the effect on global transcription determined.

2025-11-30 | GSE271330 | GEO

m6A-RIP-seq of mRNA in yw, Mettl3, Mettl14 and Hakai flies

Project description:In order to obtain a stringent m6A methylome in Drosophila adults, we performed m6A-RIP-seq in yw control (male and female), Mettl3 (male), Mettl14 (male) and Hakai (male) mutant flies. We find that the effective m6A modification, which depends on the writer complex, is mostly distributed in 5’ UTR and near start codon in Drosophila, in contrast to the mammalian system. We define a set of high-confident m6A methylation sites shared by Mettl3, Mettl14 and Hakai, indicating that Hakai is a core component of the m6A writer complex. We also find differential methylation pattern in certain loci between male and female flies.

2021-03-01 | GSE155662 | GEO

The METTL3 RNA methyltransferase regulates estrogen signalling in MCF-7 breast cancer cells

Project description:siRNAs targeting METTL3 were used to deplete METTL3 in MCF-7 breast cancer cells and the effect on basal and estrogen regulated gene expression determined.

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The role of CBLL1 (HAKAI) component of the RNA methyltransferase complex in basal and estrogen signaling in breast cancer cells (MCF-7)

Project description:The role of CBLL1 (HAKAI) component of the RNA methyltransferase complex in basal and estrogen signaling in breast cancer cells (MCF-7)

| PRJNA1130941 | ENA

Role of the E3 Ubiquitin-ligase Hakai in intestinal inflammation and cancer bowel disease

Project description:E3 ubiquitin-ligases are important for the cellular protein homeostasis and their deregulation is implicated in cancer. The E3 ubiquitin-ligase Hakai is involved in tumour progression and metastasis, through the regulation of the tumour suppressor E-cadherin. Hakai is overexpressed in colon cancer, however, the implication in colitis-associated cancer is unknown. Here, we investigated the potential role of Hakai in intestinal inflammation and cancer bowel disease.Several mouse models of colitis and associated cancer were used including AOM-DSS, acute colitis, and genetically modified mice deficient for the IL-10 gene, to analyse Hakai expression by immunohistochemistry. Interactome analysis of Hakai was performed and effect on selected protein was determined by plasmid and siRNA transfection, western-blotting, immunoprecipitation, immunofluorescence and ubiquitination assays. Lipid accumulation was assayed by oil red staining. Immunohistochemistry was also performed in inflamed colon biopsies from ulcerative colitis, Crohn's disease and colorectal cancer patients. Our results show that Hakai was downregulated in inflammatory tissues in different mouse models. Fatty Acid Synthase (FASN) protein was identified as a novel Hakai-interacting protein. Hakai induces FASN ubiquitination and degradation via lysosome, thus regulating FASN-mediated lipid accumulation. An inverse expression of FASN with Hakai expression was detected in inflammatory AOM/DSS mouse model. In conclusion, Hakai regulates FASN ubiquitination and degradation, resulting in the regulation of FASN-mediated lipid accumulation, which is associated to the development of inflammatory bowel disease. The interaction between Hakai and FASN may be an important mechanism for the homeostasis of intestinal barrier function and in the pathogenesis of this disease.

2022-10-25 | PXD029136 | Pride

The role of CBLL1 (HAKAI) component of the RNA methyltransferase complex in androgen signaling in breast cancer cells (MDA-MB-231)

Project description:The role of CBLL1 (HAKAI) component of the RNA methyltransferase complex in androgen signaling in breast cancer cells (MDA-MB-231)

| PRJNA1130945 | ENA

Two novel proteins with opposing m6A writing functions interact with HAKAI [RNA-seq]

Project description:Using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, were discovered as novel components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA. Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation. In addition, HIZ1 was found to be involved in root hair development upon auxin transport inhibition in a HAKAI-dependent manner. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ1 appears to be a HAKAI-dependent negative regulator of m6A deposition and HIZ2 is a novel and essential member of the Arabidopsis m6A writer complex.

2022-01-21 | GSE188610 | GEO

Two novel proteins with opposing m6A writing functions interact with HAKAI [meRIP-seq]

2022-01-20 | GSE174573 | GEO

Clinical and molecular significance of the RNA m6A methyltransferase complex in prostate cancer

Project description:shRNAs targeting METTL3 used to deplete METTL3 in castrate-resistant LNCaP:C42 prostate cancer cells and the effect on gene expression and splicing determined.

2023-01-04 | GSE210130 | GEO

The METTL14 component of the RNA methyltransferase complex influences estrogen signalling in MCF-7 breast cancer cells

Project description:siRNAs targeting METTL14 were used to deplete METTL14 in MCF-7 breast cancer cells and the effect on basal and estrogen regulated gene expression determined.

2025-11-30 | GSE195487 | GEO

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