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The dynamic N1-methyladenosine methylome of RNA provides insights intotheregulation oftomato fruit ripening

ABSTRACT: N1-methyladenosine (m1A) methylation is crucial for the biogenesis of diverse RNAs, thereby influencing distinct biological functions. Previous studies have established extensive m1A modifications in petunia mRNAs. However, the specifics of the abundance, distribution, and functional role of mRNA m1A modification during tomato fruit ripening remain unclear. Our study shows that m1A modifications are prevalent in tomato mRNA and are also detected in lncRNA and circRNA. The distribution of m1A peaks in mRNA segments indicates that m1A is mainly enriched at the start codon and CDS regions. By comparing wild-type (AC) tomatoes and ethylene receptor-mutant (Nr) tomatoes from the mature green (MG) stage to six days after the breaker (Br+6) stage, we observed a decrease in the overall methylation level with fruit ripening, with the Nr mutant fruits showing significantly lower methylation levels than AC fruits. Additionally, we identified differences in m1A methylation and expression levels of numerous key genes related to fruit ripening. The integrated analysis of RNA-seq and MeRIP-seq data uncovered the possible positive impact of m1A modifications on gene expression regulation. This research offers novel insights into the specific m1A methylation modifications occurring during the ripening of both wild-type AC and Nr mutant tomato fruits for the first time. Collectively, our study contributes to the growing evidence highlighting the potential significance of m1A methylation modifications in plant development and maturation.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE271369 | GEO | 2026/06/27

REPOSITORIES: GEO

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