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RNA-seq data of SR30-overexpression transgenic tomato leaves

ABSTRACT: Alternative splicing (AS) plays a vital role in plant responses to biotic and abiotic stress. The modulation of host precursor-mRNA (pre-mRNA) AS is a common pathogenic strategy among distinct pathogens. However, the underlying mechanism by which how plants respond to pathogen-modulated AS remains largely elusive. Here, we fished out a serine/arginine-rich RNA splicing factor (SR) family protein gene, SR30, that promotes tomato susceptibility to P. infestans. To explore the effect of SR30 on host pre-mRNA AS, we examine AS change in SR30 overexpression (SR30-OE) Solanum lycopersicum leaves compared to wild type using paired-end illumina RNA-seq. A large number of AS events are identified, indicating SR30 regulates the AS of tomato pre-mRNA. The overlap between differentially expression genes (DEGs) and differentially alternative splicing genes (DASGs) was low, indicating that most genes were unique to either pre-mRNA splicing or DEGs. SR30 overexpression represses the AS of tomato defense-related genes. Moreover, we also demonstrated that SR30 is upregulated and forms nuclear speckles through phase separation driven by intrinsically disordered regions (IDRs) during Phytophthora infestans. IDR2 and IDR5 deletion mutant SR30ΔIDR2&5 failed to phase separate and promote P. infestans infection. Importantly, the association between SR30 and tomato spliceosome components and the alternative splicing of host defense-related genes are altered in the SR30 in a phase-separate-dependent manner.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE271386 | GEO | 2026/07/30

REPOSITORIES: GEO

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