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Gene expression analysis during morphogenesis of Human umbilical vein endothelial cells

ABSTRACT: Gene expression profiles during the differentiation of HUVEC on matrigel were analyzed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE27160 | GEO | 2011/02/09

SECONDARY ACCESSION(S): PRJNA137401

REPOSITORIES: GEO

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Gene expression analysis during morphogenesis of Human umbilical vein endothelial cells

Project description:Gene expression profiles during the differentiation of HUVEC on matrigel were analyzed. RNA was isolated from Human umbilical vein endothelial cell during morphogenesis. HG-U133 plus 2.0 microarray (54675 human genes; Affymetrix Santa Clara, CA, USA) was performed.

2011-02-09 | E-GEOD-27160 | biostudies-arrayexpress

Expression profiling in Human Umbilical Vein Endothelial Cells (HUVEC)

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DNA methylation profiles of human umbilical vein endothelial cells (HUVECs)

Project description:Replicative senescence has major impact on function and integrity of cell preparations. DNA-methylation (DNAm) changes are continuously acquired at specific CpG dinucleotides during in vitro culture. Such modifications can be used to estimate the state of cellular senescence for quality control of cell preparations. It is however unclear how senescence-associated DNAm changes are regulated and if they occur simultaneously throughout cell populations. In this study, we analyzed global DNAm profiles of human mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVEC) to demonstrate that senescence-associated DNAm changes are overall similar in these different cell types. Subsequently, an Epigenetic-Senescence-Signature, based on six CpGs, was either analyzed by pyrosequencing or by barcoded bisulfite amplicon sequencing. There was a good correlation between predicted and real passage numbers in bulk populations of MSCs (R² = 0.67) and HUVECs (R² = 0.97).

2017-02-20 | GSE82234 | GEO

Expression profiling in Human Umbilical Vein Endothelial Cells (HUVEC)

Project description:Whole transcriptome gene expression profiling of Normal -(HUVEC) Human Umbilical Vein Endothelial Cells The HUVEC gene expression results analyzed in this study are further described in Kustermann S. et al. (2014) A real-time impedance based screening assay for drug induced vascular leakage. A NimbleGen Homo sapiens Expression Array [100718_HG18_opt_expr] study using total RNA recovered from HUVECs grown until 80% confluency. Each microarray measures the expression level of 23,611 genes using 45,033 probe sets with three 60-mer probes (PM) per probe set. Each probe set is represented once on the array.

2014-01-07 | E-GEOD-52044 | biostudies-arrayexpress

Proteomic response of human umbilical vein endothelial cells to histamine stimulation

Project description:Endothelial cells (HUVEC) were treated with histamine, the endogenous ligand of histamine receptors, for a global overview of its cellular effects. Changes of the intracellular and secreted proteomes were analyzed using shotgun proteomics. Furthermore, a selected set of proteins was quantified using parallel reaction monitoring.

2017-02-28 | MSV000080587 | MassIVE

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Project description:Identification of differentially expressed genes in HUVEC senescence and tBHP treatment.

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ChIA-PET from endothelial cell of umbilical vein (ENCSR404FPI)

Project description:CTCF ChIA-PET in HUVEC For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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ChIA-PET from endothelial cell of umbilical vein (ENCSR080OMN)

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microRNA counts from endothelial cell of umbilical vein (ENCSR223FVU)

Project description:Nanostring miRNA Array on human HUVEC For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-10-14 | GSE86804 | GEO

microRNA-seq from endothelial cell of umbilical vein (ENCSR076EJO)

Project description:RNA Evaluation human HUVEC primary cells microRNA-seq from Mortazavi For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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