Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single Cell RNA Sequencing of Radiation-Induced Esophageal Injury in Rats

ABSTRACT: We utilized single-cell RNA sequencing (scRNA-seq) to understand the effects of radiation on the cell type composition and transcriptional profiles of esophagus during self-repair after radiation-induced esophageal injury in rats.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE271766 | GEO | 2026/07/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single Cell RNA Sequencing of Radiation-Induced Esophageal Injury in Rats

Project description:Single Cell RNA Sequencing of Radiation-Induced Esophageal Injury in Rats

| PRJNA1133058 | ENA

Potential proteins identification for stent restenosis induced by esophageal benign hyperplasia in esophageal cancer

Project description:This study was conducted to explore the potential proteins associated with esophagus benign hyperplasia induced by esophageal stents.

2023-03-10 | PXD014242 | Pride

SOX15 governs a transcriptional program in human esophageal stratified epithelium and in a subset of esophageal adenocarcinomas

2015-11-09 | GSE62909 | GEO

miR-223 in esophageal adenocarcinoma carcinogenesis

Project description:miR-223 is step-wise increasingly up-regulated in the normal esophagus - Barrett's esophagus -esophageal adenocarcinoma carcinoma sequence. In this study, we aimed to determine the function of miR-223 in esophageal adenocarcinoma carcinogenesis.

2013-02-07 | GSE44120 | GEO

microRNA expression in Barrett's esophagus and esophageal adenocarcinoma

Project description:Barrett's esophagus is a metaplastic condition of the distal esophagus, characterized by the replacement of normal squamous epithelium by columnar epithelium. Patients with BE have an increased risk of developing esophageal adenocarcinoma. MicroRNAs have been implicated to be disease and tissue specific, however limited data of microRNA expression in the esophagus is available. Therefore we evaluated microRNA expression profiles of esophageal adenocarcinoma and compared these with Barrett's esophagus and normal squamous esophagus.

2012-03-15 | GSE29965 | GEO

SOX15 governs a transcriptional program in human esophageal stratified epithelium and in a subset of esophageal adenocarcinomas

Project description:We identified spatially restricted transcription factors and found SOX15 expression confined to stratified esophageal epithelium, with attenuation in Barrett's esophagus. SOX15 binds esophagus-specific loci and its loss in human esophageal cells affected esophagus-specific transcripts [RNA-Seq] Total RNA isolated from CPA control cells and CPA cells following SOX15 depletion, samples were prepared for sequencing using the TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer's instructions. 75 base pair single-end reads were sequenced on an Illumina NextSeq 500 instrument. The data include 2 independent biological replicates per genotype. [ChIP-Seq] Examine SOX15-chromatin binding in CPA cells.

2015-11-09 | E-GEOD-62909 | biostudies-arrayexpress

microRNA expression profiling of human esophageal metaplastic lesions

Project description:To test the hypothesis that there is a specific miRNA expression signature which characterizes Barrett's esophagus development and progression, we performed miRNA microarray analysis comparing normal esophageal squamous epithelium with the two different metaplastic lesions occuring within Barrett's mucosa (i.e. gastric metaplasia and intestinal metaplasia). Samples of H. pylori-related gastritis and gastric intestinal metaplasia were also considered in the definition of esophageal-specific miRNAs. miRNA microarray analysis was performed in a series of samples obtained from (a) 10 histologically-proven long-segment Barrett's esophagus patients; (b) 10 patients with H. pylori-related chronic atrophic gastritis. Overall, 10 normal esophageal squamous epithelium samples, 10 esophageal intestinal metaplasia samples, 10 esophageal gastric metaplasia samples, 10 H. pylori -related gastritis samples (no atrophic lesion detected; obtained from the antrum) and 10 gastric intestinal metaplasia samples (obtained from the antrum) were considered.

2011-01-01 | E-GEOD-24839 | biostudies-arrayexpress

Dietary zinc modulation of cancer-related inflammation genes and esophageal tumor development and prevention in rats

Project description:Weaning male Sprague-Dawley rats were divided into 2 dietary groups to form the Zn-deficient (ZD) and Zn-sufficient (ZS) groups. After 5 weeks, the animals received intragastrically, their 1st N-nitrosomethylbenzylamine dose (NMBA, an esophagus carcinogen) at 2 mg/kg body weight. Immediately after NMBA treatment, half of the ZD rats were switched to a ZS diet to form the Zn-replenished (ZR) group, the other half of ZD rats and ZS rats continued on their respective diets. Five weeks after the 1st NMBA dose, 8 ZD, 8 ZR, and 8 ZS rats were sacrificed gene expression profiling and related studies. The remaining rats received their 2nd and 3rd NMBA dose at 6 weeks and 12 weeks after the 1st NMBA dose. At 15 weeks (endpoint) after the 1st NMBA dose, all animals were sacrificed for tumor incidence analysis and gene expression profiling studies. In this study, ZD rats documented a significantly higher tumorigenic outcome than control ZS rats (tumor incidence, ZD vs ZS: 100% vs 16.6%; tumor multiplicity, 11 ± 3.8 vs 0.5 ± 0.3, P<0.001). Replenishing zinc after the first NMBA dose led to a significantly reduced tumor incidence and multiplicity in ZR vs ZD rats (incidence, 28.9% vs 100%; multiplicity, 0.6 ± 0.4 vs 11 ± 3.8, P<0.001). Expression profiling of esophageal epithelia from NMBA-treated ZD, ZR, and control ZS rats (n=4/group) was performed at 5 weeks and at 15 weeks after the 1st carcinogen dose, using Rat Genome 230 2.0 GeneChip (Affymetrix, Santa Clara, CA)

2011-01-25 | E-MTAB-428 | biostudies-arrayexpress

The effect of the HDAC inhibitor OSU-HDAC42 on gene expression in esophageal tissues and adenocarcinoma cells II

Project description:Title: In vitro and in vivo effects of the orally bioavailable phenylbutyrate-derived histone deacetylase inhibitor OSU-HDAC42 on gene expression in esophageal tissues and in esophageal adenocarcinoma cells. Histone deacetylases (HDACs) modulate nucleosomal packaging of DNA, thereby influencing gene transcription and multiple cancer-associated processes. We conducted microarray analysis on esophageal tissue from male Sprague-Dawley rats treated with OSU-HDAC42 or vehicle to assess target effect in an in vivo model utilized for EAC development. Keywords: esophageal tissue from male Sprague-Dawley rats treated with OSU-HDAC42 or vehicle

2008-03-20 | GSE9976 | GEO

Gene expression profiling of Barrett's esophagus, adenocarcinoma, esophageal squamous epithelium and squamous cell carcinoma

Project description:The aim of this study is to generate and validate biomarkers to stratify patients with Barrett’s esophagus in terms of risk for developing cancer. We studied gene expression profiling in 69 frozen specimens, consisting of esophageal squamous epithelium from 19 healthy subjects, 20 specimens from patients with Barrett’s esophagus and 21 cases of esophageal adenocarcinoma, 9 cased of esophageal squamous cell carcinoma by whole genome microarray analysis. Laser capture microdissection technique was applied to procure cells from defined regions of Barrett’s esophagus metaplasia and esophageal adenocarcinoma. Microarray results were validated by quantitative real-time polymerase chain reaction (qRT-PCR) in an independent cohort consisting of 42 cases. Furthermore, immunohistochemistry was performed using antibodies to two selected target molecules on a third independent cohort of 36 specimens, consisting of 36 cases. A total of 1176 genes were associated significantly with esophageal adenocarcinoma. The expression pattern of a 4 gene signature with the highest discriminant score based on linear discriminant analysis (GeneSpring GX10.2), was identified and validated by qRT-PCR in independent cohort. Gene expression profiling of 20 specimens of Barrett's esophagus patients, 21 specimens of adenocarcinoma patients and 19 biopsies from patients with normal esophageal squamous epithelium, 9 specimens of squamous cell carcinoma were studied.

2011-10-31 | E-GEOD-26886 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data