Escaping from a dominant‐negative KRAS using REPLACE

ABSTRACT: A dominant-negative gene therapy approach has been proposed and tested on proto-oncogene KRAS, wherein the oncogenic activity (and cell proliferation) of KRAS can be suppressed by introducing a dominant-negative KRAS allele (S17N). We employed REPLACE to conduct continuous evolution on KRAS (S17N) and examined its potential pathways for conferring resistance in this gene therapy methodology.

ORGANISM(S): Mesocricetus auratus

PROVIDER: GSE271877 | GEO | 2024/09/30

REPOSITORIES: GEO