Translation inhibition with CHX in mouse embryonic stem cells in bulk

ABSTRACT: Gene-expression noise can influence cell-fate choices across pathology and physiology. However, a crucial question persists: do regulatory proteins or pathways exist that control noise independently of mean expression levels? Our integrative approach, combining single-cell RNA sequencing with proteomics and regulator enrichment analysis, reveals 32 putative noise regulators. The approach utilizes global translation inhibition (i.e., potential protein regulators), and single-cell RNA sequencing (scRNA-seq) to quantify the changes in noise of all transcripts (i.e., potential mRNA targets). This dataset corresponds to a control bulk RNAseq experiment experiment upon translation inhibition with cycloheximide to validate the changes in mean observed with single-cell.

ORGANISM(S): Mus musculus

PROVIDER: GSE272056 | GEO | 2024/08/05

REPOSITORIES: GEO