Project description:RNA-Seq of cellular and extracellular samples from DiFi cells. The distribution of extracellular RNA among cells, sEV pellet, exomeres and supermeres is distinct.
Project description:Extracellular vesicles, including exosomes, and exomere nanoparticles, are under intense investigation for cargo that may serve as clinical biomarkers or therapeutic targets. Here, we report discovery of a new extracellular nanoparticle, termed supermeres. We performed LC/MS-MS proteomics analyses on gradient-purified sEVs, NVs, exomeres and supermeres. The proteomic profile of supermeres is clearly distinct from that of sEVs, NVs and exomeres. This study identifies a new functional nanoparticle replete with potential circulating biomarkers and therapeutic targets that can be exploited for clinical benefit in a host of diseases.
Project description:Glioblastoma is a grade IV glioma of heterogeneous nature which complicates disease pathophysiology and biomarker research. Thus, the aim of our meta-analysis is to identify long noncoding RNAs (lncRNAs) and protein coding genes (PCGs) that are differentially expressed over different glioblastoma tissue datasets. Small RNA-seq of glioblastoma tissues was also performed to identify differentially expressed microRNAs (miRNAs) relative to paired controls.
Project description:Extracellular vesicles, including exosomes, and exomere nanoparticles, are under intense investigation for cargo that may serve as clinical biomarkers or therapeutic targets. Here, we report discovery of a new extracellular nanoparticle, termed supermeres. We performed LC/MS-MS proteomics analyses on gradient-purified sEVs, NVs, exomeres and supermeres. The proteomic profile of supermeres is clearly distinct from that of sEVs, NVs and exomeres This study identifies a new functional nanoparticle replete with potential circulating biomarkers and therapeutic targets that can be exploited for clinical benefit in a host of diseases.
Project description:Advances in understanding intercellular communication through extracellular vesicles (EVs) and non-vesicular extracellular nanoparticles (NVEPs) have been limited by their cellular heterogeneity and unclear origins. Using sequential ultracentrifugation, we identified high- and low-density EVs and performed multi-omics analyses on four EV/NVEP categories, including supermeres. Our findings reveal that extracellular RNA, RNA-binding proteins, and other cellular proteins are differentially expressed among EVs and NVEPs. Notably, supermeres can penetrate the blood-brain barrier, in a manner dependent on their intact RNA and protein structure, and distribute throughout the CNS. Supermeres preferentially interact with microglia at higher levels than other CNS cell types. These interactions lead to decreased TGFβ expression, suggesting an immunomodulatory role. This study highlights supermeres as a promising platform for CNS therapeutic delivery.
