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SWI/SNF complex ATPase activity supports normal syncytiotrophoblast differentiation and shields from EVT differentiation [RNA-seq]

ABSTRACT: Syncytiotrophoblast (ST) differentiation is essential for proper formation of the placenta. Using human trophoblast stem cells as a model system, we dissect the role of SWI/SNF ATPase function during this process. We compare gene expression, chromatin accessibility, SWI/SNF occupancy and histone modifications of non-treated control cells with cell treated with BRM014, selective inhibitor of BRM and BRG1 ATPase function, induced to differentiate into ST-like cells by forskolin treatment. Gene expression was monitored at 0h, 12h, 24h and 72h of differentiation and epigenomic assays were carried out in self-renewing conditions (0h) and 24h of ST differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE272425 | GEO | 2025/12/04

REPOSITORIES: GEO

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SWI/SNF complex ATPase activity supports normal syncytiotrophoblast differentiation and shields from EVT differentiation [ChIP-seq]

Project description:Syncytiotrophoblast (ST) differentiation is essential for proper formation of the placenta. Using human trophoblast stem cells as a model system, we dissect the role of SWI/SNF ATPase function during this process. We compare gene expression, chromatin accessibility, SWI/SNF occupancy and histone modifications of non-treated control cells with cell treated with BRM014, selective inhibitor of BRM and BRG1 ATPase function, induced to differentiate into ST-like cells by forskolin treatment. Gene expression was monitored at 0h, 12h, 24h and 72h of differentiation and epigenomic assays were carried out in self-renewing conditions (0h) and 24h of ST differentiation.

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SWI/SNF complex ATPase activity supports normal syncytiotrophoblast differentiation and shields from EVT differentiation [RNA-seq]

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