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Generation of germinal-vesicle oocytes from mouse embryonic stem cells under an ovarian soma-free condition (SC3-Seq)

ABSTRACT: In vitro oogenesis provides a platform to elucidate the mechanism of oocyte development and advance reproductive medicine. The prevalent in vitro oogenesis model requires ovarian somatic cells (OSCs) to support oocyte development, yet complex three-dimensional oocyte–OSC interactions pose difficulties in systems regulation and mechanistic understanding. Here, we present an OSC-free system of in vitro oogenesis: upon optimized provision of retinoic acid and bone morphogenetic protein on feeders, mouse primordial germ cell-like cells induced from embryonic stem cells propagate robustly, and enter/progress through meiotic prophase I, generating abundant fetal oocyte-like cells at diplotene arrest. With key cytokines, signaling activators, and antioxidants, they show prominent growth and differentiate into cells comparable to germinal-vesicle oocytes in morphology, transcriptome, and histone-modification profiles, with competence to resume meiosis with germinal-vesicle breakdown. By reconstituting major phases of oogenesis with minimal components, our study creates a foundation for OSC-free in vitro oogenesis in mammals, including humans.

ORGANISM(S): Mus musculus

PROVIDER: GSE272450 | GEO | 2025/05/19

REPOSITORIES: GEO

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Generation of germinal-vesicle oocytes from mouse embryonic stem cells under an ovarian soma-free condition (CATCH-Seq)

Project description:In vitro oogenesis provides a platform to elucidate the mechanism of oocyte development and advance reproductive medicine. The prevalent in vitro oogenesis model requires ovarian somatic cells (OSCs) to support oocyte development, yet complex three-dimensional oocyte–OSC interactions pose difficulties in systems regulation and mechanistic understanding. Here, we present an OSC-free system of in vitro oogenesis: upon optimized provision of retinoic acid and bone morphogenetic protein on feeders, mouse primordial germ cell-like cells induced from embryonic stem cells propagate robustly, and enter/progress through meiotic prophase I, generating abundant fetal oocyte-like cells at diplotene arrest. With key cytokines, signaling activators, and antioxidants, they show prominent growth and differentiate into cells comparable to germinal-vesicle oocytes in morphology, transcriptome, and histone-modification profiles, with competence to resume meiosis with germinal-vesicle breakdown. By reconstituting major phases of oogenesis with minimal components, our study creates a foundation for OSC-free in vitro oogenesis in mammals, including humans.

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Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys [sc3seq_macfas]

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Polycomb function during oogenesis is required for mouse early embryonic development

Project description:This SuperSeries is composed of the following subset Series: GSE23033: Polycomb function during oogenesis is required for mouse early embryonic development (germinal vesicle oocytes) GSE28710: Polycomb function during oogenesis is required for mouse early embryonic development (2-cell embryos) Refer to individual Series

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Transcriptional profiling of somatic cells differentiation throughout oocyte competence acquisition in rainbow trout ovarian follicles.

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