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Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (overexpressed ComK)

ABSTRACT: Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N)

ORGANISM(S): Bacillus cereus ATCC 14579 Bacillus cereus

PROVIDER: GSE27265 | GEO | 2011/07/28

SECONDARY ACCESSION(S): PRJNA142107

REPOSITORIES: GEO

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Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (overexpressed ComK)

Project description:Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N) One condition design comparision of (IPTG induced overexpression construct vs IPTG induced empty plasmid) including a dye swap, 3 biological replicate

2011-07-27 | E-GEOD-27265 | biostudies-arrayexpress

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Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus

Project description:This SuperSeries is composed of the following subset Series: GSE27264: Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (induced ComK1 or ComK2) GSE27265: Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (overexpressed ComK) Refer to individual Series

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Project description:Bacillus cereus is the second leading cause of collective food poisoning in France. B. cereus is also associated with severe clinical infections leading to patient death in 10% of the cases. The emergence of B. cereus as a foodborne and opportunistic pathogen has intensified the need to distinguish strains of public health concern. In this work, by performing a screen on a large collection of B. cereus strains of varying pathogenic potential, we identified genetic determinants capable of discriminating B. cereus strains inducing negative clinical outcomes. The combination of 4 biomarkers is sufficient to accurately discern clinical strains from harmless strains. Three of the biomarkers are located on the chromosome, with a fourth one identifying a plasmid carried by most pathogenic strains. A 50 kbp region of this plasmid promotes the virulence potential of these strains and could thus be defined as a new pathogenicity island of B. cereus. These new findings help in the understanding of B. cereus pathogenic potential and complexity and may provide tools for a better assessment of the risks associated with B. cereus contamination to improve patient health and food safety.

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Effect of Combined Bifidobacterium, Lactobacillus, Enterococcus and Bacillus Cereus Tablets on Chemotherapy and Radiotherapy of Colorectal Cancer

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Project description:This project contributes to the proteomic comparison of Bacillus cereus ATCC 14579 wild-type strain and the derivative strain cured of the linear plasmid, B. cereus ATCC 14579 -pBClin15, grown in aerobiosis condition and harvested at three growth stages.

2016-07-19 | PXD002789 | Pride

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Project description:This project contributes to the proteomic comparison of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid, its mutant strain devoided of the methionine sulfoxide reductase AB (msrAB) protein and the corresponding msrAB complemented strain, all strains grown in aerobiosis condition and harvested at three growth stages.

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