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Effect of PITX2 knockdown on transcriptome of primary human trabecular meshwork cell cultures

ABSTRACT: To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factor PITX2 and to identify genes that may have roles in glaucoma. Expression profiles derived using microarrays were compared between TM control cells and cells treated with PITX2 siRNAs

ORGANISM(S): Homo sapiens

PROVIDER: GSE27275 | GEO | 2011/04/01

SECONDARY ACCESSION(S): PRJNA137141

REPOSITORIES: GEO

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Effect of PITX2 knockdown on transcriptome of primary human trabecular meshwork cell cultures

Project description:To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factor PITX2 and to identify genes that may have roles in glaucoma. Expression profiles derived using microarrays were compared between TM control cells and cells treated with PITX2 siRNAs TM1 and TM2 cells were cultured in 6 well plates were transfected with either of two PITX2 specific siRNAs or scrambled siRNA (as control). The scrambled siRNA treatment was performed in duplicate for each TM. Forty eight hours after exposure to siRNAs, cells were harvested and RNA was extracted. Considering the two siRNAs for PITX2 transcription factor, the two scrambled siRNA treatments, and the two TM primary cultures, we performed 8 array hybridizations.

2011-04-01 | E-GEOD-27275 | biostudies-arrayexpress

FOXC1 in human trabecular meshwork cells is involved in regulatory pathway that includes miR-204, MEIS2, and ITGβ1

2012-11-01 | GSE40314 | GEO

Effects of dexamethasone for primary trabecular meshwork cell gene expression

Project description:To clarify the effects of dexamethasone treatment for primary trabecular meshwork cell gene expression, which may relates to the pathophysiology of glucocorticoid-induced glaucoma Three lots (lot #2584, 3423 and 4973) of primary culture human trabecular meshwork (TM) cells were purchased from ScienCell Research Laboratories (Carlsbad, CA). The TM cells were treated with and without 100nM dexamethasone (DEX) for 14 days. Genomewide gene expression analysis was carried out using Agilent 8X60K array.

2015-01-24 | E-GEOD-65240 | biostudies-arrayexpress

Expression Profile of Genes in Trabecular Meshwork Cells Treated with Dexamethasone

Project description:Treatment with glucocorticoids is known to cause ocular hypertension in humans which can lead to steriod-induced glaucoma We used microarrays to determine changes in gene expression in two human trabecular meshwork (TM) cell isolates (TM-4 and TM-2) from the same donor that could explain the increase in ocular hypertension.

2018-12-20 | GSE124114 | GEO

Global expression analysis of the trabecular meshwork, cornea, sclera and trabecular meshwork-derived mesenchymal stem cells (TM-MSC)

Project description:Microarray study comparing the trabecular meshwork (TM) with TM-MSC, corneal and scleral tissues

2017-06-09 | GSE87526 | GEO

FOXC1 in human trabecular meshwork cells is involved in regulatory pathway that includes miR-204, MEIS2, and ITGβ1

Project description:To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factorfoxc1 and to identify genes that may have roles in glaucoma. Expression profiles derived using microarrays were compared between TM control cells and cells treated with foxc1 siRNAs. TM1 and TM2 cells were cultured in 6 well plates were transfected with either of two FOXC1 specific siRNAs or scrambled siRNA (as control). The scrambled siRNA treatment was performed in duplicate for each TM. Forty eight hours after exposure to siRNAs, cells were harvested and RNA was extracted. Considering the two siRNAs for FOXC1 transcription factor, the two scrambled siRNA treatments, and the two TM primary cultures, we performed 8 array hybridizations.

2012-11-01 | E-GEOD-40314 | biostudies-arrayexpress

Genome-wide DNA methylation in cultured human Schelmm's Canal endothelial cells and trabecular meshwork cells with or without glaucoma

Project description:Genome-wide DNA methylation profiling in cultured human Schelmm's Canal endothelial cells (SC) and trabecular meshwork (TM) cells derived from post-mortem eyes with or without glaucoma. Our study aimed to identify glaucoma-associated genes that were affected by DNA methylation.

2020-01-03 | GSE129183 | GEO

Transcription profiling of human trabecular meshwork cells after TGF-beta1 and 2 treatment

Project description:TGF-beta levels are known to increase in the aqueous humor of eye cells in patients with glaucoma. Increase TGF-beta is assumed to have a biochemical impact on the trabecular meshwork, and an increase in extracellular matrix formation, which may be responsible for decrease outflow facility of the eye. This may increase extracellular pressure, causing glaucoma. TGF-beta 1 may be the cause of abnormal accumulation of extracellular matrices in trabecular meshwork of eyes with primary open angle glaucoma. Transforming growth factor (TGF)-beta2 regulates the expression of proteoglycans in aqueous humor from human glaucomatous eyes. To identify gene expression changes as a result of TGF-beta1 and 2 treatment of human trabecular meshwork cells. We expect to see a change in expression of the proteoglycans in HTM cells as a response to TGF-beta treatment. Human Trabecular Meswork cells in the eye were bathed by aqueous humor. TM cells were removed from individuals with the following ages: 16,66,67,73, and 76. Each individual was treated with EtOH (control), TGF-beta1, or TGF-beta2. Total RNA from each individual was pooled for each chip. Technical replicates were created for each treatment type, for a total of 6 chips.

2008-06-15 | E-GEOD-7144 | biostudies-arrayexpress

Trabecular meshwork cell-derived exosomal microRNA expression

Project description:Trabecular meshwork cells in eyes with glaucoma aquire mesenchymal phenotypes. The types of microRNAs in exosomes may differ between static and glaucomatous status and their effects on aqueous humor regulation are still uknown. We used microarrays to identify the differential microRNA expression related to interaction between trabecular meshwork cells and Schlemm's canal endothelial cells.

2021-11-17 | GSE175464 | GEO

Effect of glucocorticoid chemical structure and receptor potency on human trabecular meshwork cells

Project description:Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells. Gene expression changes of human trabecular meshwork cells, TM 86 and TM 93, due to treatment with dexamethasone (Dex), fluocinolone acetonide (FA), and triamcinolone acetonide (TA).

2010-02-15 | GSE16643 | GEO

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