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Transcriptomic response to substrate and temperature in two thermophilic fungi, Myceliophthora thermophila and Thielavia terrestris, and a related mesophile, Chaetomium globosum

ABSTRACT: The thermophilic filamentous fungi Myceliophthora thermophila (Sporotrichum thermophile) and Thielavia terrestris are proficient decomposers of cellulose, suggesting that they will be a rich source of thermostable industrial enzymes for lignocellulose degradation. To identify the genes and proteins involved in this process, we explored the transcriptomes of M. thermophila and T. terrestris growing at 45 ºC on either glucose, alfalfa, or barley straw by short-read sequencing of extracted mRNA. To better understand the adaptations that allow these fungi to grow at elevated temperatures, we compared their transcriptomes when growing at 34C to their transcritomes at 45C, and also to the transcriptome of the related fungus Chaetomium globosum, which does not grow at 45C.

ORGANISM(S): Chaetomium globosum Thermothielavioides terrestris Thermothelomyces thermophilus

PROVIDER: GSE27323 | GEO | 2011/08/08

SECONDARY ACCESSION(S): PRJNA137175

REPOSITORIES: GEO

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Transcriptomic response to substrate and temperature in two thermophilic fungi, Myceliophthora thermophila and Thielavia terrestris, and a related mesophile, Chaetomium globosum

Project description:The thermophilic filamentous fungi Myceliophthora thermophila (Sporotrichum thermophile) and Thielavia terrestris are proficient decomposers of cellulose, suggesting that they will be a rich source of thermostable industrial enzymes for lignocellulose degradation. To identify the genes and proteins involved in this process, we explored the transcriptomes of M. thermophila and T. terrestris growing at 45 ºC on either glucose, alfalfa, or barley straw by short-read sequencing of extracted mRNA. To better understand the adaptations that allow these fungi to grow at elevated temperatures, we compared their transcriptomes when growing at 34C to their transcritomes at 45C, and also to the transcriptome of the related fungus Chaetomium globosum, which does not grow at 45C. RNA was extracted from cultures in early growth stage growing with glucose, alfalfa, or barley straw as carbon source at 34C or 45C (M. thermophila and T. terrestris); duplicate cultures were sampled in some conditions.

2011-08-08 | E-GEOD-27323 | biostudies-arrayexpress

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Transcriptomic response to substrate and temperature in two thermophilic fungi, Myceliophthora thermophila and Thielavia terrestris, and a related mesophile, Chaetomium globosum

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| PRJNA137175 | ENA

Transcriptional landscape of Myceliophthora thermophila responding to soluble starch and the role of regulator AmyR on polysaccharide degradation

Project description:Thermophilic fungus Myceliophthora thermophila with great capacity for polysaccharides degradation is attractive to be engineered into a cell factory to produce chemicals and biofuels directly from renewable polysaccharides such as starch. Understanding the molecular mechanism of starch degradation of the fungi would be helpful. To date, there has been no transcriptome analysis on starch in thermophilic fungi. In this study, we performed the transcriptomic profile of M. thermophila responding to soluble starch, and a 342-gene set was identified as “starch regulon”, including the major amylolytic enzyme (Mycth_72393), which was verified thereafter as the most important such hydrolase for starch degradation in this fungus. Moreover, the function of key amylolytic enzyme regulator AmyR in M. thermophila was evaluated by analyzing the performance of its deletion mutant using our CRISPR/Cas9 system, which showed significantly decreased amylase activity and poor growth on starch. Additionally, deletion of amyR led to resistance to carbon catabolite repression (CCR) and enhanced cellulases production. Our study provides an insight into understanding the molecular basis of starch degradation in this thermophilic fungus, and will accelerate the fungal strain rational engineering for starch-based biochemical production.

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Reconstruction and analysis genome-scale metabolic model of thermophilic fungus Myceliophthora thermophila

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