Decoding the interplay between m6A modification and stress granule stability by live-cell imaging

ABSTRACT: We present a spatiotemporal m6A imaging system (SMIS) that can monitor the m6A modification and the translation of mRNAs with high specificity and sensitivity in a single live cell. The SMIS system contains a m6A-modified reporter mRNA and fluorescence resonance energy transfer (FRET) biosensors. To quantify the methylation viariation achieved at m6A sites in the reporter mRNA after STM2457 treatment, a classical METTL3 inhibitor, we performed m6ACE-seq to measure the relative m6A level with single-base-resolusion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE273366 | GEO | 2024/10/11

REPOSITORIES: GEO