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Human Growth Hormone (GH) responsive genes in peripheral blood mononuclear cells (PBMC)

ABSTRACT: Biomarkers of responsiveness to treatment with recombinant human GH (rhGH) have only poorly been described. We aimed at identifying biomarkes of rhGH treatment in children. Our aim was to identify the genes in response to rhGH in human PBMC, that can be used as Biomarkes of growth prediction.

ORGANISM(S): Homo sapiens

PROVIDER: GSE27446 | GEO | 2012/12/01

SECONDARY ACCESSION(S): PRJNA137073

REPOSITORIES: GEO

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human Growth Hormone (GH) responsive genes in peripheral blood mononuclear cells (PBMC)

Project description:Biomarkers of responsiveness to treatment with recombinant human GH (rhGH) have only poorly been described. We aimed at identifying biomarkes of rhGH treatment in children. Our aim was to identify the genes in response to rhGH in human PBMC, that can be used as Biomarkes of growth prediction. Blood was obtained from pediatric patients before and 4 days after the growth hormone therapy. RNA was isolated from the PBMC and subsequently used to perform the whole genome cDNA microarrays. We used the type-1 microarray strategy (RNA isolated before the hormone treatment as a control, and RNA isolated after rhGH treatment). Microarrays represent PBMC RNA obtained from growth hormone treated patients (green channel = baseline, red channel = GH tretmanet for 4 days). Stanford cDNA microarrays were used. Only genes corresponding to spots with fluorescence signals of 1.5-fold over array background in both, the experimental channel (Cy5) and the reference channel (Cy3) and only genes with at least 80% interpretable data in the 9 microarray experiments were considered. Of note, the Cy5/Cy3 ratios on the arrays correspond directly to the fold up-or down-regulation of gene transcription following rhGH treatment. Subsequently, the Cy5/Cy3 ratios on arrays were log2 transformed for further analyses and the dataset was further reduced by considering only those genes with at least a 2-fold up- or down- regulation of transcription in least 3 of the 9 microarray experiments. Microarray experiments and genes were then organized by unsupervised hierarchical clustering using the Pearson correlation metric and average linkage clustering.

2012-12-01 | E-GEOD-27446 | biostudies-arrayexpress

Growth Hormone (GH) Treatment of 3T3-F442A Adipocytes

Project description:Expression profiling of 3T3-F442A adipocytes treated with growth hormone (GH, 500 nM) or vehicle (DMEM + 1% BSA) control for 30 min., 4 hr., or 48 hr in three independent experiments. Chronic GH treatment induces metabolic changes consistent with insulin resistance in 3T3-F442A adipocytes. Keywords: time-course

2005-12-20 | GSE2120 | GEO

Sex-dependent and growth hormone (GH)-dependent gene expression

Project description:A series of dual-channel gene expression profiles obtained using MWG Rat 5K microarrays (~5535 unique rat genes) and MWG Rat Liver microarrays (~1353 unique rat genes with 999 of these genes also represented on the Rat 5K microarray) was used to examine the sex-dependent and GH-dependent differences in gene expression in adult rat liver. This series is comprised of 8 randomly chosen pairings of independent male and female rat liver cDNA samples and 8 randomly chosen pairings of independent male and continuous GH-treated male rat liver cDNA samples, totaling 16 samples. Half of the samples were hybridized to the MWG Rat 5K microarrays and the other half were hybridized to the MWG Rat Liver microarrays. Comparison of the set of sex-dependent genes with the set of GH-responsive genes shows that 90% of male-dominant genes are suppressed in male rats treated with a female pattern of GH. Approximately 73% of female-dominant genes were up-regulated in the continuous GH-treated male rats. Keywords = Growth hormone Keywords = liver sexual dimorphism Keywords = cytochrome P450 Keywords = liver gene expression Keywords = dual channel cDNA microarray Keywords: repeat sample

2004-03-27 | GSE1146 | GEO

Transcription profiling of mouse 3T3-F442A adipocytes treated with or without growth hormone (GH) to identify a mechanism for regulation of activating transcription factor 3 by GH

2007-09-21 | E-GEOD-2120 | biostudies-arrayexpress

Proteomic changes of the liver in the absence of growth hormone (GH) actions

Project description:The liver is a central target organ of growth hormone (GH), which stimulates the synthesis of insulin-like growth factor 1 (IGF1) and affects multiple biochemical pathways. A systematic multi-omics analysis of GH actions in the liver has not been performed. GH receptor (GHR) deficiency is a unique model for studying consequences of lacking GH actions. In this study, we used molecular profiling techniques to capture a broad spectrum of these effects in the liver of a clinically relevant large animal model.

2020-03-30 | PXD017671 | Pride

Growth Hormone (GH) -secreting Pituitary Adenoma

Project description:We profiled the somatic landscape of 21 growth hormone (GH) -secreting pituitary adenomas using somatic copy-number alteration (SCNA), whole-genome sequencing (WGS), bisulfate sequencing, and transcriptome approaches. See details in Valimaki et al. Genetic and epigenetic characterization of growth hormone (GH) - secreting pituitary tumors. Manuscript in preparation, 2019.

| EGAS00001003488 | EGA

Continuous GH treatment of old rat liver

Project description:Effect of continuous GH treatment on old rat liver. Male rats, 2-year-old, were treated with vehicle or human GH (0.34 microgram/gram body weight) for 3 weeks. Keywords: response of old rat liver to growth hormone

2005-07-19 | GSE2962 | GEO

Continuous GH treatment of old rat liver

Project description:Effect of continuous GH treatment on old rat liver. Male rats, 2-year-old, were treated with vehicle or human GH (0.34 microgram/gram body weight) for 3 weeks. Comparison of treated and untreated rats. Individual RNA were used for 4 microarray hybridization. Dye swapped.

2010-06-09 | E-GEOD-2962 | biostudies-arrayexpress

Growth Hormone (GH) -secreting Pituitary Adenoma

Project description:Data from 12 fresh-frozen somatotropinomas and their corresponding blood samples. Details are given in Valimaki et al. Whole-genome sequencing of Growth Hormone (GH) -secreting Pituitary Adenoma. Provisionally accepted, 2015.

| EGAS00001001293 | EGA

Response of liver-expressed genes (including lincRNAs) to continuous growth hormone (GH) infusion

Project description:Gene expression in livers of adult male mice subjected to continuous GH infusion using Alzet osmotic minipumps for 1, 4 or 7 days was assayed by RNA-seq, as part of a study of growth hormone regulation of hepatic lincRNAs (PMID:26459762) and protein-coding genes (PMID:28694329).

2015-10-21 | GSE66008 | GEO

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