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Gene expression profile of olive oil- and ozonated olive oil-treated A375 human melanoma cell lines

ABSTRACT: We analyzed the effect of ozonated olive oil on gene expression of A375 human melanoma cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE274530 | GEO | 2024/08/14

REPOSITORIES: GEO

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Transcriptomic profiile of A375 melanoma cell treated with Olive oil and Ozoanted olive oil

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Gene expression profile of olive oil- and ozonated olive oil-treated A375 human melanoma cell lines

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Project description:Olive oil is protective against risk factors for cardiovascular and cancer diseases. A nutrigenomic approach was performed to assess whether olive oil, the main fat of the Mediterranean diet modifies the gene expression in human peripheral blood mononuclear cells. Six healthy male volunteers ingested, at fasting state, 50 ml of olive oil, and continued with the same olive oil as a source of raw fat (25ml/day) during 3 weeks. Prior to intervention a 1-week washout period with sunflower oil as the only source of fat was followed. During the 3 days before, and on the intervention day, a very low phenolic compound diet was followed. At baseline (0h), at post ingestion (6h), and at fasting state after 3 weeks of sustained consumption of olive oil total RNA was isolated from PBMC. Gene expression was evaluated by microarray and verified by qRT-PCR. Keywords: Olive oil, gene expression, single dose, sustained consumption

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