Dataset Information

Regulation of undruggable c-MYC with controlled photothermal therapy for efficient cancer immunotherapy

ABSTRACT: Cancer immunotherapies have revolutionized the treatment of metastatic cancers however most patients cannot benefit from current immunotherapies due to a suppressive tumor immune microenvironment (TIME). Photothermal therapy (PTT) with nanoparticles (NPs) as photothermal conversion agents has shown potential to induce immunogenic cell death (ICD) by remodeling TIME. PTT with different temperatures have been applied and mild temperature-PTT was recently claimed as the most promising approach. However, the optimal PTT temperature and related mechanisms in activation of immunotherapy have remained unclear. In the present study, black porous silicon (BPSi) NPs coated with polyethylene glycol and cancer cell membranes were utilized as photothermal agents. These NPs have good biocompatibility, high photothermal conversion efficacy, and homotypic tumor targeting ability. It was found that the PTT with the BPSi NPs could efficiently induce ICD, which was largely affected by the applied temperature in PTT. As compared to the mild temperature (46°C) and high temperature (54 °C) PTT, medium temperature PTT at 50 °C was the optimal treatment to induce effective ICD in vitro and inhibit tumor growth in vivo. Furthermore, it was unveiled that PTT could efficiently regulate undruggable c-MYC by tuning temperatures. c-MYC was upregulated by PTT at 46°C while downregulated by PTT at 50 °C. The activities of MYC-induced pathways such as p53 were also significantly enhanced. These findings not only discover the mechanisms in activating immunotherapy with NPs-based PTT but also suggest new directions for developing more potent therapy regimens to treat metastatic tumors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE275258 | GEO | 2025/08/26

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Purpose: We aimed at i) obtaining insight into how a thermophile organism reacts to cold stress, and ii) evaluating the impact of HGT candidates on the acclimation process to temperature decrease. Methods: The experimental design followed a temperature shift timeline: after two weeks of cultivation at 42°C, constant illumination (90 μE) and constant shaking (160 rpm) in photoautotrophic conditions the first sampling took place (Hot_T48_1) and the cultures of G.sulphuraria were swiftly moved to 28°C ( = cold temperature). "Cold" samples were taken after 3h (Cold_T3_2), 12h (Cold_T12_3) and 48h (Cold_T48_4). After cold treatment at 28°C for 48 hours, the G. sulphuraria was then switched to 46°C for 48 hours (="Hot"). Again, samples were taken after 3h (Hot_T3_5), 12h (Hot_T12_6) and 48h (Hot_T48_7). Altogether, a 48h temperature timeshift at 28°C and successive recovery at 46°C were targeted for sampling. Results: Galdieria sulphuraria is a unicellular red alga that lives in hot, acidic, toxic metal-rich, volcanic environments, where few other organisms survive. Its genome harbours up to 5% of genes most likely acquired through horizontal gene transfer. These genes probably contributed to G. sulphuraria’s adaptation to its extreme habitats, resulting in today’s polyextremophilic traits. Here, we applied RNA-sequencing to obtain insights into the acclimation of a thermophilic organism towards temperatures below its growth optimum and to study how horizontally acquired genes contribute to cold acclimation. A decrease in growth temperature from 42 °C/46 °C to 28 °C resulted in an upregulation of ribosome biosynthesis, while excreted proteins, probably components of the cell wall, were downregulated. Photosynthesis was suppressed at cold temperatures, and transcript abundances indicated that C-metabolism switched from gluconeogenesis to glycogen degradation. Folate cycle and S-adenosylmethionine cycle (one-carbon metabolism) were transcriptionally upregulated, probably to drive the biosynthesis of betaine. All these cold-induced changes in gene expression were reversible upon temperature increase. Numerous genes acquired by horizontal gene transfer displayed pronounced temperature-dependent expression changes, corroborating the view that these genes contributed to adaptive evolution in G. sulphuraria.

Dataset Information

Regulation of undruggable c-MYC with controlled photothermal therapy for efficient cancer immunotherapy

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets