Regulation of Immature Porcine Sertoli cell proliferation by tRNAGly-GCC and tRNAGlu-CTC-derived small RNAs

ABSTRACT: Male fertility is contingent upon spermatogenesis. Sertoli cells, as somatic cells within the seminiferous tubules of the testis, create a specific immune environment conducive to the development of spermatocytes and spermatids, thereby ensuring the smooth progression of spermatogenesis. The proliferation and maturation of Sertoli cells are regulated by non-coding small RNAs. In this study, we explored the expression characteristics and functions of tsRNA in porcine Sertoli cells using small RNA sequencing, immunofluorescence staining, LC-MS/MS, and other techniques. The results of small RNA sequencing indicated that 97.5% of tsRNA in mature porcine Sertoli cells originated from the 5' end of tRNA, with the cleavage site primarily located near the antisense codon, and the length distributed between 31-35 nt. The abundance of tsRNA in porcine Sertoli cells accounted for 4.37% of the total small RNA, which was higher than that observed in spermatogonia and pachytene spermatocytes. Fourteen tsRNA families were found to be highly enriched in the mature Sertoli cells of porcine testis. Three tsRNAs, specifically Gly GCC 2-1, Glu CTC 1-1, and Glu CTC 1-6, were highly enriched in both mature and immature Sertoli cells. Furthermore, YTHDF1 was confirmed as the target gene of Gly GCC 2-1, while CDK1 was identified as the target gene of Glu CTC 1-1. Both Gly GCC 2-1 and Glu CTC 1-1 were shown to reduce the viability of Sertoli cells and inhibit their proliferation. The findings of this study provide a foundational understanding for the development of porcine Sertoli cells and offer a theoretical reference for the regulation of spermatogenesis.

ORGANISM(S): Sus scrofa

PROVIDER: GSE275578 | GEO | 2026/08/23

REPOSITORIES: GEO