TCR repertoire analysis of donor-derived and iTEC-supported thymocytes

ABSTRACT: We analyzed the TCRb repertoires of CD4 and CD8 single-positive thymocytes isolated from a pediatric donor, as well as CD4 and CD8 single-positive thymocytes matured in vitro from the double-positive stage in 3D organoid co-culture with iPSC-derived thymic epithelial cells (iTECs). Our analysis shows that iTECs are capable of positively selecting thymocytes with diverse TCR repertoires in vitro.

ORGANISM(S): Homo sapiens

PROVIDER: GSE275815 | GEO | 2025/07/10

REPOSITORIES: GEO